Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for separating and purifying rhamnolipid

A rhamnolipid, separation and purification technology, applied in the field of separation and purification, can solve the problems of multiple extractions, difficulty in separation, and unavoidable crossover of components, and achieve the effects of good repeatability, low purity and simple process

Active Publication Date: 2010-07-28
DAQING HUALI ENERGY BIOLOGICAL TECH
View PDF3 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main defect of the extraction method is that the number of extractions is too many, emulsification is easy to occur, and further separation is difficult. At the same time, the crossover of components in the extraction process is difficult to avoid, and the purity of the product is affected.
Arino S (Arino S, Marchal R, Vandecasteele J P. Identification and production of a rhamnolipidic biosurfactant by a Pseudomonas species. Appl Microbiol Biotechnol, 1996, 45: 162-168), Manso Pajarron (A. Manso Pajarron, C.G. De Koster, W. Heerma, M. Schmidt, J. Haverkamp, ​​Glycoconjugate Journal, Structure identification of natural rhamnolipid mixtures by fast atom bombardment tandem mass spectrometry 1993, 10: 219-226.), Rendell (Rendell, N.B., Taylor, G.W., Somerville, M., Todd, H., Wilson, R.and Cole, P.J.Characterization of Pseudomonas rhamnolipids.Biochim.Biophys.Acta, 1990,1045,189-193) reported a method for separating rhamnolipids by TLC, which can separate pigments out, but due to the small amount of TLC sample loading, mass production cannot be achieved
Patent CN200610136880.0 adopts freeze-drying method to separate and purify according to the influence of temperature and pH value on the solubility of rhamnolipids, but as the pH value and temperature decrease, the solubility of some impurities will also decrease and together with rhamnolipids Separated, the obtained rhamnolipids have lower purity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for separating and purifying rhamnolipid
  • Method for separating and purifying rhamnolipid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for separating and purifying rhamnolipids, the method comprising the following steps:

[0025] (1) Centrifuge the rhamnolipid-containing fermentation broth at 5000r / min for 30min, adjust the pH of the obtained supernatant to 2.0 with 6mol / L hydrochloric acid, leave it overnight at 4°C and centrifuge at 5000r / min for 30min, collect the precipitate Utilize diethyl ether to extract, after combining organic phase, blow-dry and freeze-dry to obtain rhamnolipid crude product 0.5g, adopt the mixed organic solvent obtained by mixing chloroform and methanol at a volume ratio of 25:1, take the mixed organic solvent 1.0 mL dissolves the rhamnolipid crude product to obtain a dissolved sample;

[0026] (2) Add the dissolved sample obtained in step (1) to a 200-mesh silica gel column 200mL of the first organic solvent obtained by mixing chloroform and methanol at a volume ratio of 25:1 was added to a silica gel column to elute the dissolved sample, and then chloroform and ...

Embodiment 2

[0030] A method for separating and purifying rhamnolipids, the method comprising the following steps:

[0031] (1) Centrifuge the rhamnolipid-containing fermentation broth at 5000r / min for 30min, adjust the pH of the obtained supernatant to 1.5 with 6mol / L hydrochloric acid, leave it overnight at 4°C and centrifuge at 5000r / min for 30min, collect the precipitate Utilize ethyl ether to extract, combine the organic phases and then blow dry and dry to obtain 6.0 g of rhamnolipid crude product, adopt the mixed organic solvent obtained by mixing chloroform and methanol at a volume ratio of 20:1, and take 12.0 mL of the mixed organic solvent Dissolving the rhamnolipid crude product to obtain a dissolved sample, the amount of the dissolved sample is 2% of the column volume of the silica gel column;

[0032] (2) Add the dissolved sample obtained in step (1) to a 300-mesh silica gel column, the diameter of the silica gel column is 3cm, and the length and diameter ratio of the silica ge...

Embodiment 3

[0036] A method for separating and purifying rhamnolipids, the method comprising the following steps:

[0037] (1) Centrifuge the rhamnolipid-containing fermentation broth at 5000r / min for 30min, adjust the pH of the obtained supernatant to 1.8 with 6mol / L hydrochloric acid, leave it overnight at 4°C and centrifuge at 5000r / min for 30min, collect the precipitate Utilize ethyl ether to extract, after merging the organic phases, blow-dry and freeze-dry to obtain 2.0Kg of rhamnolipid crude product, adopt the mixed organic solvent obtained by mixing chloroform and methanol in a volume ratio of 30:1, take the mixed organic solvent 4.0 L dissolves the rhamnolipid crude product to obtain a dissolved sample, and the amount of dissolved sample is 2% of the column volume of the silica gel column;

[0038] (2) Add the dissolved sample obtained in step (1) to a 300-mesh silica gel column. The diameter of the silica gel column is 30 cm, and the aspect ratio is 10:1. Chloroform and methanol...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to a method for separating and purifying rhamnolipid, which comprises the following steps: dissolving the crude product of rhamnolipid in mixed organic solvent; adding the dissolved sample to a silica gel column; eluting the dissolved sample by using the mixed organic solvent; collecting the eluent and separating pigments from the eluent to obtain rhamnolipid solution; and finally, subjecting the rhamnolipid solution to reduced-pressure distillation and concentration to obtain the purified rhamnolipid. Compared with the prior art, the invention overcomes the defects in the prior art and has the characteristics of simple operation, high sample-loading capacity and high separation efficiency. Therefore, the method of the invention constitutes an extraction method advantageous in simple process and simple, economical and effective application and suitable for industrialized batch production.

Description

technical field [0001] The invention relates to a separation and purification method, in particular to a separation and purification method of rhamnolipid. Background technique [0002] Rhamnolipids are some amphiphilic compound molecules with certain surface activity and a combination of hydrophilic and hydrophobic structures produced by microorganisms under certain conditions. Compared with chemical surfactants, rhamnolipids have the characteristics of non-toxic, harmless, degradable, good biocompatibility, resistance to extreme harsh conditions, good selectivity and specificity, so they are used in oil exploration, environmental management, It has potential application value in food industry, paper industry, cosmetics, biomedicine and other fields. However, since the production cost of rhamnolipids is 3-6 times higher than that of chemical surfactants (the cost of separation, extraction and concentration in the middle and lower reaches accounts for 60%-70% of the product...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07H15/04C07H1/06C07H1/08
Inventor 牟伯中杨世忠刘金峰王丹丹
Owner DAQING HUALI ENERGY BIOLOGICAL TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com