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Arginine hybrid cell-penetrating peptide and application thereof

A technology of arginine and membrane-penetrating peptides, which is applied in the direction of peptides, preparations for in vivo tests, and non-effective ingredients of polymer compounds, and can solve problems such as expensive, weak targeting, and unsuitable for commercial production.

Inactive Publication Date: 2010-07-21
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods have their own advantages, there are also some disadvantages: low introduction rate, strong stimulation to cells and even cell death, poor targeting
However, these non-natural peptides are difficult to prepare due to the high price of their monomers or have not been commercialized, and are not suitable for commercial production

Method used

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  • Arginine hybrid cell-penetrating peptide and application thereof
  • Arginine hybrid cell-penetrating peptide and application thereof
  • Arginine hybrid cell-penetrating peptide and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] The non-natural D-arginine (r=D-arginine) and the natural L-arginine (R=L-arginine) are alternately linked into an octameric peptide, named (rR) 4 . As a control group, an all-L-arginine octamer was designed and named as R 8 . R 8 The membrane penetration efficiency is much higher than that of traditional TAT 49-57 protein, but because it also relies on endocytosis, it is trapped in the vesicle after penetrating the membrane. The two membrane-penetrating peptides were labeled with the common non-toxic fluorescein FITC, respectively.

[0027] In the experiments of detecting cell-penetrating peptides, different research groups often have different results in detecting the penetration of membrane-penetrating peptides into cells. The experimental procedures and results of different research groups show that slight changes in experimental conditions will affect the results of cell experiments. Studies have shown that if the method of fixing cells first and then observing...

Embodiment 2

[0047] According to the above steps, we have successively carried out verification tests of arginine cell penetrating peptides with other structures, including (rR) 3 , (rR) 4 r, etc., the various conclusions drawn from these experiments are also consistent with (rR) 4 The situation is basically the same, which shows that it is constructed by hybridization of non-natural D-arginine (r) and natural L-arginine (R), and the structure is (r) n (R) m Arginine cells can carry markers and cargo molecules into the cell, and have the characteristics of high membrane penetration efficiency, strong resistance to enzymatic hydrolysis, specific labeling of living cell nuclei, and low cytotoxicity.

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Abstract

The invention discloses arginine hybrid cell-penetrating peptide and application thereof, belonging to the field of biological molecule. The cell-penetrating peptide is prepared by hybrid construction from non-natural D-arginine (r) and natural L-arginine (R) and has a structure of (r) n (R) m, wherein r and R can be randomly arranged in the structure, n and m are the number of amino acid residues, the sum of n and m is greater than or equal to 4 and less than or equal to 12, n is greater than or equal to 1 and less than or equal to 11, and m is greater than or equal to 1 and less than or equal to 11; and the N end and the C end of the cell-penetrating peptide can be singly or simultaneously connected with different markers or cargo molecules and carry the markers and the cargo molecules into cells. The cell-penetrating peptide can carry the markers and the cargo molecules into the cells with high efficiency and low cytotoxicity, simultaneously has very strong enzymolysis resistant and serum degradation resistant capacities, and can escape from vesicles corpuscles after entering the cells.

Description

technical field [0001] The present invention belongs to the field of biomolecules. Background technique [0002] With the development of bioengineering, genomics, biology and organic chemistry, various types of new drug molecules, such as: short peptides (peptides), monoclonal antibody proteins (monoclonal antibodies), antisense oligonucleotides (antisense oligonucleotides) , ribozymes (ribozymes), catalytic DNA (catalytic DNA) and so on have been developed. These molecules of great biological and medical significance (including traditional plasmids and fusion proteins, of course) often limit their in vivo applications due to their excessive molecular weight and their own hydrophilic properties. Due to the obstruction of the biomembrane system, it is difficult for these molecules to enter the cells of the target organs and tissues to exert their effects. To address these problems, different technologies have been developed, and they all have their own advantages and disadv...

Claims

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Application Information

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IPC IPC(8): C07K5/11C07K7/06C07K7/08A61K49/14A61K47/42A61K47/64
Inventor 张玉慧骆清铭马严
Owner HUAZHONG UNIV OF SCI & TECH
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