Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Invitro tissue cultivation method of hepatocyte of mammal

A technology of mammals and culture methods, applied in the direction of animal cells, vertebrate cells, artificial cell constructs, etc., can solve the problems of inconvenient transplantation, tissue formation, difficult to culture cells, and lack of liver cell function

Inactive Publication Date: 2010-07-14
苑国忠
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are more and more methods for in vitro culture of cells, and they are also more and more advanced, but the problem is that it is difficult to cultivate a considerable number of cells in a certain period of time during the in vitro culture of primary hepatocytes, so that transplantation back to the body cannot achieve function of liver cells
Moreover, the hepatocytes obtained by the above method can be used in in vitro bioreactors, and it is not convenient to transplant into the body to form organized cells.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] 1. Cell Culture Substrate Preparation

[0022] The mixture of 20-40% collagen or 20-40% silk fibroin and 60-80% polylactic acid (molecular weight 10000-150000 Daltons) is dissolved in dichloromethane to make electrospinning with a mass concentration of 6-8%. The liquid is woven into a flat cylinder with a diameter of 1-2cm and a height of 3-4mm through electrospinning technology. Dry in vacuo to remove residual dichloromethane. The matrix volume is 93-96% microporous and 4-7% substrate. The diameter of the pores is between 80um and 500um, and the micropores are connected to each other. Among them, about 4% of the pores have an average diameter of 80-110 μm; about 6% of the pores have an average diameter of 111-120 μm; about 7% of the pores have an average diameter of 121-130 μm; about 5% of the pores have an average diameter of 131-260 μm; About 5% of the pores have an average diameter of 261-300 μm; about 15% of the pores have an average diameter of 301-340 μm; abou...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
heightaaaaaaaaaa
pore sizeaaaaaaaaaa
Login to View More

Abstract

The invention relates to an invitro tissue cultivation method of hepatocyte of a mammal, adopting a hepatocyte culture medium with a three-dimensional porous structure of static tissue. The medium has a diameter of 1-2mm and a thickness of 3-4mm; the medium is provided with holes differing in size, wherein the diameter of the hole ranges from 80mum-500mum, and holes are communicated with each other. The method ensures that the hepatocyte of human or animal is rapidly multiplied during culture in vitro and keeps the cell biology and function of the hepatocyte cell.

Description

technical field [0001] The invention relates to the tissue culture of hepatocytes in vitro by using a novel specific culture substrate. Background technique [0002] The liver is an important organ of the human body, and liver lesions, such as liver cirrhosis and liver failure, will endanger the lives of patients. Clinically, liver transplantation is performed on patients with advanced liver lesions to prolong the life of patients. Due to the source of liver donors, many patients with advanced liver disease end their lives because they cannot wait for a donor. Even if a donor is obtained, the patient who receives a liver transplant will bear the economic burden of life-long drug immunosuppression in later life. With the development of tissue engineering, new therapeutic means offer patients the possibility to improve and revive liver function. [0003] Tissue engineering integrates the basic principles, basic theories, basic technologies and basic methods of engineering a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071A61L27/38A61L27/26A61L27/24A61L27/22A61L27/18A61L27/56
Inventor 苑国忠
Owner 苑国忠
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com