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Method for immobilizing petroleum degradation bacteria by taking surface root hair of reed as vector

A technology of petroleum degrading bacteria and roots, which is applied in the direction of being fixed on or in an inorganic carrier, can solve the problems of affecting the production capacity of wetlands and the accumulation of pollutants, and achieve the effects of low cost, good purification effect and simple process

Inactive Publication Date: 2010-06-30
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

By prolonging the hydraulic retention time of sewage in the wetland in order to improve the purification effect of natural wetlands, pollutants will accumulate in the soil due to excessive residence time, thereby affecting the production capacity of wetlands.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The concentration of petroleum-degrading bacteria was taken as 5.70×10 6 Add 0.1g of sodium alginate to 50mL of each / mL suspension, mix well and drop evenly on reed root hairs. After immobilization for 5 hours, immerse the immobilized carrier of petroleum-degrading bacteria into the micro-pollution simulated Liaohe estuary oil pollution Water (100mL seawater + 300mL tap water + 1g diesel). After 20 days at a room temperature of 16°C, the purification effect of the oil-degrading bacteria-immobilized carrier on oil-polluted water is 60%.

Embodiment 2

[0029] The concentration of petroleum-degrading bacteria was taken as 3.82×10 6 pcs / mL suspension 50mL, add 0.1g sodium alginate, mix evenly and drop evenly on reed roots, after immobilization for 5 hours, immerse the immobilized carrier of petroleum-degrading bacteria into slightly polluted water simulating oil pollution in Liaohe Estuary (100mL seawater + 300mL tap water + 1g diesel). After 20 days at a room temperature of 16°C, the purification effect of the petroleum-degrading bacteria-immobilized carrier on slightly polluted water is 59%.

Embodiment 3

[0031] The concentration of petroleum-degrading bacteria was taken as 2.85×10 6 pcs / mL suspension 50mL, add 0.1g sodium alginate, mix evenly and drop evenly on reed roots, after immobilization for 5 hours, immerse the immobilized carrier of petroleum-degrading bacteria into slightly polluted water simulating oil pollution in Liaohe Estuary (100mL seawater + 300mL tap water + 1g diesel). After 20 days at a room temperature of 16°C, the purification effect of the petroleum-degrading bacteria-immobilized carrier on slightly polluted water is 58%.

[0032] Visible, even at low concentrations of 2.85×10 6 Units / mL and lower temperature, the petroleum-degrading bacteria suspension of the present invention still has a strong purification ability and effect on petroleum sewage, which is greatly conducive to the large-scale popularization and use of reed wetlands.

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Abstract

The invention relates to a method for immobilizing petroleum degradation bacteria by taking surface root hairs of reeds as vectors, which intensifies the decontamination capacity of a natural wetland for a petroleum polluted water body. The method is characterized by comprising the following steps of: firstly, screening out purified petroleum degradation bacteria strains by soil polluted by petroleum; then inoculating the purified petroleum degradation bacteria strains to a liquid enriched medium so as to culture petroleum degradation bacteria turbid liquid; finally adding sodium alginate to the prepared petroleum degradation bacteria turbid liquid, mixing, and then uniformly spraying to the root hairs of the reeds so as to achieve immobilization 5 hours later, i.e. immobilizing the petroleum degradation bacteria on the surface root hairs of the wetland reeds. The invention has the advantages of extensive material sources, simple process, low cost and good purifying effect, can achieve very good degradation effect even if the petroleum degradation bacteria turbid liquid with lower concentration is used and can be popularized and used on a large area of a reed wetland.

Description

technical field [0001] The invention belongs to the field of strengthening the cleaning function of natural wetlands, and in particular relates to a method for immobilizing petroleum-degrading bacteria using reed surface roots as carriers. Background technique [0002] At present, more than half of my country's surface water bodies are polluted to varying degrees, seriously affecting people's production and life. There are many methods to purify polluted surface water bodies, which are mainly divided into physical purification, chemical purification and biological purification. Among them, the physical purification method uses physical and mechanical methods to artificially purify the polluted surface water body. This type of method is simple in process, but it is expensive and treats the symptoms rather than the root cause. The chemical purification method mainly refers to adding chemical agents to the polluted water body, and through the chemical reaction between the agen...

Claims

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Application Information

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IPC IPC(8): C12N11/14
Inventor 田伟君白洁张竹圆赵阳国
Owner OCEAN UNIV OF CHINA
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