Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Chlorella cell wall disruption method

A technology of chlorella and ball milling tank, which is applied in the field of chlorella wall breaking, can solve the problems of high cost, clear liquid pollution, and destruction of active components of chlorella, and achieves the effect of low cost, low equipment requirements and simple operation

Inactive Publication Date: 2010-06-30
张炳泉
View PDF0 Cites 23 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chlorella wall-breaking technology has been difficult to solve for a long time.
From the 1960s to the present, people have studied many physical, chemical and even biological methods to break the wall of chlorella, such as ultrasound, high pressure, heating, transgenic, strong acid and strong alkali, etc., but these methods may destroy the chlorella. active ingredients, or will cause re-contamination of the clear liquid after breaking the wall, or the cost is high, making chlorella products a luxury product

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Use freeze-thaw method to break the wall of chlorella

[0038] Step 1: Add purified water with pH=9 to the concentrated chlorella culture solution and wash it 3 times to remove impurities;

[0039] Step 2: Put the chlorella concentrate obtained after washing into the refrigerator for quick freezing, and then place it in a warm water bath at 37°C to melt;

[0040] Repeat step 2 for 2 to 3 times to obtain a green mixture.

[0041] Observed under a microscope, it can be seen that the chlorella algae wall is cracked and the contents partially overflow, and the degree of wall breakage reaches the first level.

Embodiment 2

[0043] The chlorella wall breaking method of the present invention

[0044] Step 1: Add purified water with pH=9 to the concentrated chlorella culture solution and wash it 3 times to remove impurities;

[0045] Step 2: Put the chlorella concentrate obtained after washing into the refrigerator for quick freezing, and then place it in a warm water bath at 37°C to melt;

[0046] Repeat step 2 three times to obtain the chlorella concentrate with the cell wall initially broken;

[0047] Step 3: Put the initially broken chlorella concentrate into a grinder and grind for 30 minutes to obtain green algae mud.

[0048] Observed under a microscope, it can be seen that all the contents of Chlorella overflowed in a mushy state, that is, the degree of wall breakage reached the third level.

Embodiment 3

[0050] The chlorella wall breaking method of the present invention

[0051] Step 1: Add purified water with pH=9 to the concentrated chlorella culture solution and wash it 3 times to remove impurities;

[0052] Step 2: Put the chlorella concentrate obtained after washing into the refrigerator for quick freezing, and then place it in a warm water bath at 37°C to melt;

[0053] Repeat step 2 twice to obtain the chlorella concentrate with the cell wall initially broken;

[0054] Step 3: Put the initially broken chlorella concentrate in a grinder for 1 hour to obtain green algae mud.

[0055] Observed under a microscope, it can be seen that all the contents of Chlorella overflowed in a mushy state, that is, the degree of wall breakage reached the third level.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a chlorella cell wall disruption method which includes the following steps: step a: washing chlorella concentrate culture fluid through clear water to remove the culture solution therein so as to get chlorella concentrate liquid; step b: freezing the chlorella concentrate liquid quickly, then heating to enable the frozen chlorella concentrate liquid to thaw quickly; and step c: mechanically grinding the chlorella concentrate liquid obtained from the step b. The chlorella cell wall disruption method is simple in operation, low in cost and low in requirement for the equipment, and the cell wall of the chlorella can be disrupted at high efficiency. Meanwhile, high temperature and high pressure are not needed and no chemicals are necessary to be added, so the nutrition of the chlorella can be preserved to the maximum and green, safe and edible chlorella with the cell wall disrupted can be obtained.

Description

Technical field [0001] The invention relates to a chlorella wall breaking method, in particular to a chlorella breaking method for preparing edible chlorella broken wall. Background technique [0002] When deep-sea fish DHA caused people's anxiety due to pollution problems, microalgae DHA as a new type of nutrient food ingredient and nutrient supplement has attracted widespread attention and favor from all over the world. Because microalgae can be cultivated artificially, it is pollution-free and has high safety in use. With the continuous improvement of human food structure, microalgae health-care series of food and beverages will be favored by people with their unique charm. The research and development of microalgae health food has broad market prospects. [0003] Chlorella, also known as green algae, has a history that can be traced back to the Cambrian period 540 million years ago. At present, no animal or plant in the world can grow 4 to 8 times every 24 hours like chlorell...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A23L1/337A23L1/29A23L17/60A23L33/00
Inventor 张炳泉
Owner 张炳泉
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com