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Probiotic active product and preparation method thereof

A probiotic and active technology, applied in the field of active probiotic products and their manufacturing, can solve problems such as microbial imbalance and affect the survival of beneficial bacteria, and achieve the effects of cost reduction and survival rate improvement.

Active Publication Date: 2010-06-16
中健联(无锡)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some antibiotics can kill harmful bacteria in the intestinal tract, they can also affect the survival of beneficial bacteria and cause microecological imbalance

Method used

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  • Probiotic active product and preparation method thereof
  • Probiotic active product and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029]Take 5 parts of milk powder, 5 parts of soybean powder and 90 parts of water to prepare a culture medium. The prepared culture medium is sterilized at 110°C for 20 minutes, and then cooled to 35°C; the percentage by weight is 3% for bifidobacteria and 97% for the medium Inoculate the strains at a ratio of 37°C for 20 hours, when the acidity is 55°T, cool the fermentation broth to 5°C, and end the cultivation; take another sterilized medium, cool it to 45°C, press 2% Lactobacillus bulgaricus, 2% Streptococcus thermophilus, 96% of the culture medium was inoculated with strains, and then cultured at 43°C for 3 hours. At this time, the acidity was 60°T, and the fermentation liquid was cooled to 5°C to end the cultivation .

[0030] Centrifuge the above two cultured fermentation liquids at a temperature of 15°C and a rotational speed of 3500r / min for 15 minutes, pour off the supernatant, collect the obtained bacteria, and then add 20 parts of trehalose as a freeze-drying prot...

Embodiment 2

[0032] Take 7 parts of milk powder, 7 parts of soybean powder and 86 parts of water to prepare a culture medium. The prepared culture medium is sterilized at 115°C for 15 minutes, and cooled to 37°C; the percentage by weight is 2% for bifidobacteria and 98% for the culture medium Proportionally inoculated strains, and then cultured at 37°C for 15 hours. At this time, the acidity was 50°T. The fermentation broth was cooled to 9°C to end the cultivation; Bacillus 1%, Streptococcus thermius 1%, 98% of the culture medium was inoculated with strains, and then cultured at 43°C for 5 hours. At this time, the acidity was 50°T. The fermentation broth was cooled to 9°C to end the cultivation.

[0033] Centrifuge the above two cultured fermentation liquids at a temperature of 20°C and a rotational speed of 3000r / min for 10 minutes, pour off the supernatant, collect the obtained bacteria, and then add 10 parts of trehalose as a freeze-drying protective agent. Put it in a vacuum freeze dry...

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Abstract

The invention relates to a probiotic active product and a preparation method thereof. The probiotic active product comprises the following components in part by weight: 30 to 50 parts of bifidobacterium, 25 to 35 parts of lactobacillus Bulgaria, 25 to 35 parts of streptococcus thermophilus and 8 to 25 parts of trehalose. The preparation method for the probiotic active product comprises the following steps: respectively inoculating the bifidobacterium, the lactobacillus Bulgaria and the streptococcus thermophilus in a culture medium, and fermenting to obtain thallus; and then preparing the obtained thallus into the freeze dry bacterial powder probiotic active product by adopting a vacuum refrigerating and drying mode. The method prevents the activity of probiotic from being affected by oxygen in the production process by using an anaerobic technique and takes trehalose as a protective agent, so that the product containing high-activity probiotic is produced and the high activity of lactobacilli in a preservation period is maintained; meanwhile, the production process is simple and easy, and the manufacturing cost is low.

Description

technical field [0001] The invention belongs to the technical field of nutritious food, and specifically relates to a probiotic active product made by anaerobic technology based on human probiotics and a manufacturing method thereof. Background technique [0002] With the development of the economy and the improvement of people's living standards, people pay more and more attention to their own health. The environment, air, diet, mental stress, living habits and many other factors can all affect the internal environment of the human body. Especially the environment in the human intestinal tract is composed of harmful bacteria and beneficial bacteria, usually 90% of beneficial bacteria and 10% of harmful bacteria. Scientific research shows that people's physical immunity, mental state, and complexion are closely related to the excretion of toxins in their own metabolism. If you often have diarrhea and constipation, it indicates that his gastrointestinal function is disorder...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/09A23L33/125A23L33/135
Inventor 徐赟姣丁东新
Owner 中健联(无锡)生物科技有限公司
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