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Albizzia julibrissin extracting solution and application thereof

The technology of extracting solution and Albizia Julibrissin is applied to the prevention and treatment of tobacco common mosaic virus disease, the preparation of Albizia Julibrissin extract, and the application field of tobacco disease control, can solve the problems such as no reports, achieve strong antiviral activity, and be easy to scale The effect of production and large application value

Inactive Publication Date: 2010-06-16
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many studies on Albizia julibrissin in medicine and health care. For example, Albizia julienne has the effects of calming the nerves and relieving depression, promoting blood circulation and relieving pain, and appetizing Qi, but there is no report on the invention of plant disease control, especially tobacco leaf disease control.

Method used

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  • Albizzia julibrissin extracting solution and application thereof
  • Albizzia julibrissin extracting solution and application thereof
  • Albizzia julibrissin extracting solution and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0017] Preparation Example 1: Preparation Method 1 of Albizia Julibrissin Extract

[0018] (1) After pulverizing the leaves of Albizia Julibrissin, mix them with phosphate buffer according to the ratio of weight to volume of 10g / 20ml, shake and mix well, extract at 3°C ​​for 12h, then centrifuge at 4°C / 10000g for 25min, discard the precipitate , collecting the supernatant to obtain the crude extract of Albizia Julibrissin. The phosphate buffer (recipe: 61ml 0.02mol / L disodium hydrogen phosphate+31ml 0.02mol / L sodium dihydrogen phosphate+0.85% solid sodium chloride) is 150mmol / L, pH7.0;

[0019] (2) Add solid ammonium sulfate to the Albizia Julibrissin crude extract obtained in step (1) to reach 40% saturation, let stand for 3h, then centrifuge at 4°C and 10000g for 15min, discard the precipitate, and collect the supernatant;

[0020] (3) Add solid ammonium sulfate to the supernatant obtained in step (2) to reach 80% saturation, let it stand for 2h, then centrifuge at 4°C and ...

preparation Embodiment 2

[0021] Preparation Example 2: Preparation Method 2 of Albizia Julibrissin Extract

[0022] (1) After pulverizing the leaves of Albizia Julibrissin, mix them with phosphate buffer at a ratio of 10g / 20ml by weight to volume, shake and mix well, extract at 6°C for 8h, then centrifuge at 4°C / 10000g for 15min, and discard the precipitate , collecting the supernatant to obtain the crude extract of Albizia Julibrissin. The phosphate buffer is 150mmol / L, pH7.0;

[0023] (2) Add solid ammonium sulfate to the Albizia Julibrissin crude extract obtained in step (1) to reach 40% saturation, let stand for 1h, then centrifuge at 4°C and 10000g for 25min, discard the precipitate, and collect the supernatant;

[0024] (3) Add solid ammonium sulfate to the supernatant obtained in step (2) to reach 80% saturation, let stand for 2h, then centrifuge at 4°C and 10000g for 20min, discard the supernatant, and dissolve the precipitate with phosphate The buffer solution (the same as that used in step...

Embodiment 4

[0032] Example 4 Albizia Julibrissin Extract Anti-Tobacco Mosaic Virus Activity Test 2

[0033] After diluting the Albizia Julibrissin extract 10 times and 40 times, the activity detection was carried out according to the method in Example 3, with 3-4 leaves per plant, and repeated three times.

[0034] Table 2 Anti-tobacco mosaic virus experiment results of extracts of Albizia Julibrissin at different concentrations

[0035]

[0036] The results showed that the 10-fold and 40-fold dilutions of Albizia Julibrissin extract used in this example had 83.5% and 69.3% inhibition of TMV respectively.

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Abstract

The invention relates to an albizzia julibrissin extracting solution and application thereof. Albizzia julibrissin is taken as raw materials, and the albizzia julibrissin extracting solution is prepared by the following method: crushing albizzia julibrissin leaves, then leaching and centrifuging the powder of the albizzia julibrissin in buffer solution of phosphate to obtain albizzia julibrissin crude extracting solution; and adding solid ammonium sulfate into the albizzia julibrissin crude extracting solution, performing salting-out on the mixture twice, and then dialyzing and desalting to obtain the albizzia julibrissin extracting solution. The albizzia julibrissin extracting solution is applied to tobacco and has the advantages that: (1) the albizzia julibrissin extracting solution is natural plant extracting solution which has no harm to human bodies, poultry and environment and the characteristics of high efficiency, low toxicity and no residue; (2) the albizzia julibrissin extracting solution has strong antiviral activity to tobacco mosaic virus (TMV) and has the inhibitive rate of over 90 percent; (3) the albizzia julibrissin extracting solution can be effectively used for preventing and controlling the tobacco mosaic virus during a tobacco seedling culturing period, a transplanting period, a granulation period and a vigorous growth period; and (4) the albizzia julibrissin extracting solution has simple preparation method, low cost, easy scale production and great application value.

Description

technical field [0001] The invention belongs to the field of plant disease prevention and control, and provides a technical method for the prevention and treatment of tobacco common mosaic virus (TMV), in particular to the preparation of an Albizia Julibrissin extract and the application of the extract in the prevention and treatment of tobacco diseases. Background technique [0002] Plant virus diseases have brought considerable harm to agricultural production, and almost every crop is affected by 2-3 major virus diseases. Plant viruses are absolutely parasitic to host cells. Once a plant is infected, it will be in a state of life-long damage, making its control more difficult and often causing serious losses to agricultural production. [0003] According to reports, in 2006, my country's tobacco virus disease occurred in an area of ​​2.4063 million mu, resulting in a loss of 28.1072 million kilograms of output and 251.6185 million yuan in output value, accounting for 37.7%...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/20A01P1/00
Inventor 尹启生金光辉杨军宋纪真金立锋李锋王信民罗朝鹏薛超群
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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