Method for screening low enrichment cadmium peanut varieties by fluorescent quantitative PCR technique

Abstract

The invention provides a method for screening low enrichment cadmium peanut varieties by a fluorescent quantitative PCR technique, which relates to a method for screening the low enrichment cadmium peanut varieties. The method comprises the following steps of: 1, culturing peanut seedlings by using nutrient solution; 2, designing specific different cadmium treatment levels; 3, performing a method for detecting and analyzing expression differences of AhMT2a genes of peanuts; and 4, screening cadmium sensitive varieties by using the method for detecting the expression differences of the genes by using the fluorescent quantitative PCR technique according to the screening bases of culturing the peanut seedlings by using the nutrient solution, and using sequences of the AhMT2a genes of leaf blades of the seedlings. The method has the advantages of simple detection method, no need of probe design, low cost and good universality, and can perform melting curve analysis to detect the specificity of amplified reactions.

Description

Technical field: [0001] The invention relates to a method for screening low-enrichment cadmium peanut varieties, in particular to a method for screening low-enrichment cadmium peanut varieties by fluorescent quantitative PCR technology. Background technique: [0002] Peanut is one of the four major oil crops in the world. It is also the oil crop and economic crop with the highest yield per unit area and total output in my country. The planting area exceeds 5 million hectares, and its output accounts for 1 / 2 of the total output of oil crops and 45% of the world's total peanut output. %, has reached more than 14.5 million tons. Over the years, peanut has been the oil crop with the highest foreign exchange earning in my country. At present, the main challenges faced by my country's peanut production are aflatoxin pollution, pesticide residues (mainly butylhydrazine pollution) and high cadmium (Cd) content in seeds. Cd has attracted much attention due to its high toxicity and h...

AI Technical Summary

Problems solved by technology

However, the conventional screening methods include harvesting full peanuts through potted cadmium treatment, conducting atomic absorption measurement of cadmium content in seeds and measuring the differences in physiological responses of different peanut varieties to cadmium stress. However, there are many varieties, long growth cycles, and cumbersome operations. High cost, troublesome analysis and processing

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