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Method for producing microorganisms for processing ammonia-containing wastewater

A technology of microbial flora and production method, applied in the field of environmental microorganisms, can solve the problems of low scale production efficiency and high product price, and achieve the effects of large production volume, good use performance and high removal load

Active Publication Date: 2012-05-30
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although there are some technologies on the cultivation of nitrifying bacteria at present, the large-scale production efficiency is low, the product price is relatively expensive, and can only be used to treat ammonia nitrogen wastewater with low concentration such as lower than 500mg / L, such as the nitrification disclosed in Chinese patent CN1509991A The bacterial agent is mainly used for the removal of ammonia nitrogen in seawater aquaculture and high-density aquaculture in industrialized circulating seawater

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 The expanded cultivation of microbial flora for treating ammonia-containing wastewater

[0026] The microbial flora enriched and domesticated in this laboratory was used as the inoculum for mass production. First, the inoculum was inoculated into a 10L reactor for primary scale-up cultivation of the bacteria. The initial biomass was 2g, the concentration of ammonia nitrogen in the culture solution was 800mg / L, the COD was 46mg / L, and the volume of the initial culture solution was 5L; Ammonium chloride solution with an ammonia nitrogen concentration of 10-15g / L was added every 24 to 48 hours. After the ammonium salt was supplemented, the ammonia nitrogen concentration in the culture solution was higher than 700mg / L. After 7 days of cultivation, the MLSS was 1.5g / L. Since then, the removal rate of ammonia nitrogen has decreased, and the aeration was stopped. After natural sedimentation, the supernatant was removed, and the bacteria were transferred to a 60L reac...

Embodiment 2

[0027] Example 2 Expansion of microbial flora for processing ammonia-containing wastewater

[0028] The microbial flora enriched and domesticated in this laboratory was used as the inoculum for mass production. First, the inoculum was inoculated into a 10L reactor for the first-stage amplified culture of the bacteria. The initial culture medium volume was 5L. After inoculation, the MLSS was 0.9g / L, the influent ammonia nitrogen concentration was 1000mg / L, and the COD was 80mg / L. ; During the cultivation process, ammonium sulfate solution with an ammonia nitrogen concentration of 12-25g / L was supplemented every 24 to 48 hours. After the ammonia salt was supplemented, the ammonia nitrogen concentration in the culture solution was higher than 900mg / L. After 7 days of cultivation, the MLSS was 2.3g / L. L, since then the removal rate of ammonia nitrogen has decreased, stop the aeration, remove the supernatant after natural sedimentation, and transfer the bacterium to a 100L reactor ...

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Abstract

The invention discloses a method for producing microorganisms for processing ammonia-containing wastewater. Inoculums are put into a bioreactor for carrying out three-level amplification culture step by step, wherein the inoculums are microorganisms for processing the ammonia-containing wastewater and having strong endurance capacity; the first-level culture adopts a way of supplementing ammoniumsalts in batch, the second-level culture and the third-level culture adopt ways of alternately supplementing materials in batch and changing water in batch; and the microorganisms are collected afterthe three-level amplification to be used for a high ammonia-nitrogen containing wastewater processing system or storage for later use. The method has the advantages of high culture efficiency, large production quantity and the like, and is suitable for largely and commercially producing the microorganisms for processing the ammonia-containing wastewater. The microorganisms can effectively processlow-COD and high ammonia-nitrogen containing wastewater, such as catalyst production wastewater and the like.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a batch production method for treating ammonia-containing waste water with microbial flora, which can realize the industrialization and commercialization of ammonia nitrogen-removing microbial flora. Background technique [0002] Biological denitrification of wastewater includes two processes of nitrification and denitrification. The microorganisms involved in nitrification are mainly nitrifying bacteria, which are typical autotrophic bacteria that obtain energy from the process of biological oxidation of ammonia nitrogen to nitrate nitrogen. Generally, inorganic carbon is used as the Carbon source, so the metabolism time is long, the growth is relatively slow, it belongs to Gram-negative bacteria, the content of peptidoglycan in the cell wall is low, the content of protein and fat is high, and it is sensitive to environmental changes. Receptivity is relati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F101/38C02F101/16C12R1/01
Inventor 高会杰黎元生李志瑞唐似茵尹佩林
Owner CHINA PETROLEUM & CHEM CORP
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