Parental human lung adenocarcinoma cell line with high potential of bone metastasis

A technology of lung adenocarcinoma cell line and lung adenocarcinoma, applied in the field of parental Chinese lung adenocarcinoma cell line, can solve the problems of limited collection of clinical specimens, delayed research progress in diagnosis and treatment of bone metastasis of lung cancer, etc., to improve the level of clinical diagnosis and treatment, deepen the The effect of knowing

Inactive Publication Date: 2012-05-23
SHANGHAI CHEST HOSPITAL
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Problems solved by technology

Since the occurrence of lung cancer bone metastases is mostly seen in patients with advanced surgical contraindications, the clinical specimens are limited, which largely delays the progress of research on the diagnosis and treatment of lung cancer bone metastases

Method used

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  • Parental human lung adenocarcinoma cell line with high potential of bone metastasis
  • Parental human lung adenocarcinoma cell line with high potential of bone metastasis
  • Parental human lung adenocarcinoma cell line with high potential of bone metastasis

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Experimental program
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Effect test

Embodiment 1

[0040] The pericardial effusion of the patient was taken as the primary cell culture according to the conventional method, and the experimental animal immunodeficiency mice (nude mice, SCID mice, NIH-III BNX mice) were used to form metastasis after injection of the experimental mice. After the bone metastases were identified by imaging agent tracing, the diseased tissues were cultured in vitro to obtain a subset of lung cancer cells with bone metastases. The above cycle was repeated to finally obtain a parental human lung adenocarcinoma cell line with high potential for bone metastases, which was named CPA-Y1.

[0041] Analyze the biological characteristics of the above bone metastatic cells to determine the parental human lung adenocarcinoma cell lines with high potential for bone metastases, including ultrastructural comparison; cell population doubling time; karyotype analysis; tail vein, subcutaneous Injection site tumorigenicity, metastasis and specificity.

[0042] The r...

Embodiment 2

[0044] The established human lung adenocarcinoma cells (CPA-Y1) 75×104 were injected into the blood system of immunodeficiency mice, and metastasis was formed after the injection of experimental mice. After the radioisotope bone imaging agent traced and confirmed the bone metastasis, Cultured lesion tissue in vitro to obtain bone metastatic lung cancer cell subsets, repeated the above cycle, weighed every other day after two weeks, and performed radionuclide bone imaging every week from the third week. The results confirmed that the parental cells had high potential bone metastases Lung cancer cell CPA-Y1.

[0045] The medium involved is RPMI1640 or DMEM medium containing 10% calf or fetal bovine serum, pH=7, culture temperature is 37°C, 5% CO 2 .

Embodiment 3

[0047] Immunodeficient mice were injected with CPA-Y1 through the left lung, with 750,000 to 800,000 cells / mouse. Two weeks later, the lower limbs of the mice were paralyzed. After three weeks, bone metastases were found to be 90% as determined by nuclide bone imaging and pathology. And only 10% have lung metastases at the same time. Comprehensively using gene chips, real-time quantitative PCR, Western Blotting and other technologies to further analyze the functional genome related to bone metastasis of lung cancer and the genes related to bone metastasis, the results show that the CPA-Y1 cells can be used to explore the diagnosis and treatment of human lung cancer. Provide a technical platform for bone metastasis and systematic research on multiple metastases of lung cancer. At the same time, it can further deepen the understanding of the molecular mechanism of bone metastasis of lung cancer, and provide an effective molecular detection method for early diagnosis of bone meta...

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Abstract

The invention belongs to the field of micro-organic zooblast system. The invention adopts pericardial effusion of human lung adenocarcinoma patients as a material for building the system and establishes a parental human lung adenocarcinoma cell line with high potential of bone metastasis through cell cultivation, which is called CPA-Y1; and the preservation number of the parental human lung adenocarcinoma cell line is CGMCC No.2521. The established cell line can provide reference data for carrying out early diagnose on human lung adenocarcinoma bone metastases; relevant gene chip technology can be further established; and treatment effect of various medicaments comprising chemical medicaments, biological medicaments, immune medicaments, Chinese medicaments, radioactive medicaments and the like can be evaluated and researched. The parental human lung adenocarcinoma cell line provides a technological platform for searching and diagnosing human lung adenocarcinoma bone metastases and systematically studying multiple lung cancer metastasis. Meanwhile, the parental human lung adenocarcinoma cell line can be used for further analyzing functional genome related to lung cancer bone metastasis and genes related to bone metastasis to provide an effective molecular detection method for early diagnosis and evaluation of treatment effect of bone metastasis.

Description

technical field [0001] The invention belongs to the fields of biotechnology and microbial animal cell lines, and specifically relates to a parental Chinese lung adenocarcinoma cell line with high potential for bone metastasis. Background technique [0002] According to statistics at home and abroad, lung cancer is one of the malignant diseases that seriously threaten the survival and development of human beings, and has become the number one killer of tumor diseases. In 2006, about 174,470 new cases of lung cancer were diagnosed in the United States, accounting for about 13% (male) and 12% (female) of all tumors. 31% (men) and 26% (women), the death rate ranks first. There are more than 50,000 new cases of lung cancer in Japan every year, and the mortality rate is nearly 90%. According to the survey of malignant tumors in Shanghai in recent years, lung cancer (21.56%) ranks first in the incidence of malignant tumors in Shanghai males, and it is second only to breast cancer...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/08
Inventor 杨顺芳
Owner SHANGHAI CHEST HOSPITAL
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