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Method for appraising salinity tolerance potential by using stress resistance of isolated leaves

An isolated leaf and stress-resistant technology is applied in the field of identifying the salt tolerance potential of poplar, which can solve the problems of being affected by environmental factors, high cost of molecular identification, and long growth period of poplar, achieving low cost, saving time and manpower, speeding up effect of steps

Inactive Publication Date: 2012-11-21
TIANJIN CITY AGRI BIO TECH RES CENT
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  • Description
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Problems solved by technology

However, the growth period of poplar is long, and it takes a long time to evaluate the growth shape, phenological traits, growth period, etc., which is time-consuming and labor-intensive. The cost of molecular identification is high, which consumes manpower and material resources. Impact

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  • Method for appraising salinity tolerance potential by using stress resistance of isolated leaves
  • Method for appraising salinity tolerance potential by using stress resistance of isolated leaves

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Embodiment Construction

[0018] Aiming at the problems of long time, many materials and low work efficiency in identifying the salt-tolerant potential of plants by traditional techniques, the inventors conducted a lot of research work, and finally established a new method for identifying the salt-tolerant potential of poplar by using the stress-resistant ability of detached leaves. method. Based on the relative conductivity of detached leaves at a certain salt concentration, the salt-tolerant potential of different genotypes can be quickly and accurately determined, thereby screening excellent genotypes with high salt-tolerant potential, and eliminating a large number of useless genotypes in a short period of time. materials, and then bred new varieties of poplar with good salt tolerance.

[0019] The present invention aims to provide a scientific and efficient salt-tolerant rapid identification method, through which a large number of salt-intolerant genotypes are eliminated, the combination of salt-t...

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Abstract

The invention discloses a method for appraising salinity tolerance potential by using stress resistance of isolated leaves. In the method, the leaves directly absorb NaCl, membrane permeability of leaf cells can be destroyed, and the magnitude of relative conductivity can precisely reflect the salinity tolerance of different poplar leaf cells. The membranes of poplar leaf cells are destroyed in high-salt condition, destruction degrees of cell membranes with different salinity tolerance are different, the more serious the destruction degree, the more the exudate in the cells; the destruction degrees of leaf cell membranes are judged on the basis of the relative conductivity of the isolated leaves in saline solution, thus judging genetypes of poplar with different salinity tolerance. The relative conductivity of the isolated leaves which are processed a certain time in high saline solution can rapidly and precisely reflect the salinity tolerance of the genetypes. By using the appraisingtechnology, the salinity tolerance potential of the genetypes can be precisely judged by processing the leaves under certain salt concentration for 6-12min, thus saving labor and time, and having extremely high working efficiency and very wide application prospect.

Description

technical field [0001] The invention belongs to the plant detection of biotechnology, in particular to a method for identifying the salt-tolerant potential of poplar by utilizing the stress resistance ability of detached leaves. Background technique [0002] The saline-alkali soil is widely distributed in the world, and it accompanies the process of desertification, which not only causes the destruction of resources and the huge loss of agricultural production, but also poses a threat to the biosphere and ecological environment. With the growth of the world's population and the improvement of people's living standards, land and fresh water resources are becoming more and more scarce, and land salinization has become one of the increasingly serious environmental problems. The development and utilization of vast medium-to-severe saline-alkali land can not only prevent further aggravation of soil salinization, but also expand the planting area of ​​farmland, and solve the contr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/04
Inventor 郏艳红杨迎霞张颖刘仲齐
Owner TIANJIN CITY AGRI BIO TECH RES CENT
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