Submerged fermentation culture method of straw mushroom liquid strain and culture medium thereof

Abstract

The invention relates to a submerged fermentation culture method of straw mushroom liquid strains and culture mediums thereof. The invention solves the technical defects of the prior art, such as long mother species propagation time, poor hypha quality, long fungus ball production time, different fungus ball sizes, higher production cost and the like. The method is used for the submerged fermentation culture of the straw mushroom liquid strains. The method comprises the following steps: 1. carrying out mother species culture in a special culture medium of straw mushroom mother species; 2. carrying out transition culture in a special culture medium of straw mushroom stocks; 3. homogenizing the stocks to obtain homogeneous succulent strains; 4. carrying out primary shaking culture and amplification in a shaking liquid culture medium; 5. carrying out secondary shaking culture and amplification; and 6. carrying out fermentation treatment in a submerged fermentation liquid culture medium to obtain the finished products of the straw mushroom liquid strains. The invention relates to the special culture medium of straw mushroom mother species, the special culture medium of straw mushroom stocks, the shaking liquid culture medium and the submerged fermentation liquid culture medium.

Description

Technical field: [0001] The invention relates to a submerged fermentation culture method and a culture medium thereof, in particular to a submerged fermentation culture method and a culture medium of straw mushroom liquid strains. Background technique: [0002] Straw mushrooms have been produced using solid strains. In the annual production of Straw Mushroom factory, because the production cycle of Straw Mushroom is very short, so the demand for strains is very large, so solid strains can not meet the needs of the annual production of Straw Mushroom factory. There are still many problems in the currently used fermented culture technology of straw mushroom liquid strains, specifically: (1) use a general-purpose medium to propagate the mother species, so the breeding time of the mother species is longer and the mycelium quality is not high; (2) more The solid strains cultured with the mother seed medium are directly cultured in a first-level shake flask without homogenization...

AI Technical Summary

Problems solved by technology

In the annual production of Straw Mushroom factory, due to the extremely short production cycle of Straw Mushroom, the demand for strains is very large, so solid strains cannot meet the needs of Straw Mushroom factory annual production.

There are still many problems in the currently used fermented culture technology of straw mushroom liquid strains, specifically: (1) use a general-purpose medium to propagate the mother species, so the breeding time of the mother species is longer and the mycelium quality is not high; (2) more The solid strains cultured with the mother seed medium are directly cultured in a first-level shake flask without homogenization treatment. In this way, the production time of the bacterial balls is long and the size of the bacterial balls is different, which is not conducive to the growth of the bacterial balls and affects the growth of the grass. The quality of mushroom liquid spawn

(3) The medium used for liquid strains contains a large amount of glucose, sucrose and other ingredients, which makes the cultivation environment and the cultivation environment produce a great contrast. Finished product production