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Method for tissue culture and rapid propagation of thornless safflower

A technology of tissue culture and thorn safflower, which is applied in botany equipment and methods, soilless cultivation, horticultural methods, etc., can solve problems such as unclear influence, and achieve simple and practical methods, strong stability, and low requirements for equipment Effect

Inactive Publication Date: 2010-04-21
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some studies have been carried out abroad, in actual research, so far, because the tissue culture work of safflower is not as extensive and in-depth as other plants, the factors that affect tissue regeneration in other plants such as genotype, sex and The effects of various factors such as age, tissue source, and physiological state are not very clear in safflower, so further research is needed

Method used

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  • Method for tissue culture and rapid propagation of thornless safflower
  • Method for tissue culture and rapid propagation of thornless safflower
  • Method for tissue culture and rapid propagation of thornless safflower

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] a. Pick the plump Yumin thornless safflower seeds, and then sterilize the seeds with 0.1% mercury liters on a clean bench for 15 minutes, disinfect the surface with 75% alcohol for 1 minute, and rinse with sterile water 5 times;

[0021] b. Put the treated seeds in step a into the germination medium 1 / 2MS, the temperature is 23±1℃, the light is 2000lx, and the time is 16h / d for cultivation;

[0022] c. Cut off the leaves and roots of the 7-day seedling in step b, and insert the 1cm stem into the medium MS+6-BA 1.0mg / L+TDZ 0.5mg / L+PP 333 In 0.5mg / L, placed in temperature 23±1℃, light 2000lx, time 16h / d for propagation culture;

[0023] d. Cut off the 1.0cm axillary buds of the seedlings expanded in step c, insert them into the medium 1 / 2MS+NAA 0.2mg / L, and place them at a temperature of 23±1℃, a light 2000lx, and a time of 16h / d Induced rooting;

[0024] e. For the seedlings with a height of 8 cm in step d, first open the sealing film and smelt the seedlings in the greenhouse f...

Embodiment 2

[0026] a. Pick the plump Yumin thornless safflower seeds, then sterilize the seeds with 0.1% mercury liters on a clean bench for 17 minutes, disinfect the surface with 75% alcohol for 30 seconds, and rinse with sterile water 4 times;

[0027] b. Put the treated seeds in step a into the germination medium 1 / 2MS, the temperature is 23±1℃, the light is 2000lx, and the time is 16h / d for cultivation;

[0028] c. Cut off the leaves and roots of the 8d seedling in step b, and insert the 1.5cm stem into the medium MS+6-BA 1.0mg / L+TDZ 0.3mg / L+PP 333 In 2.0mg / L, placed in temperature 23±1℃, light 2000lx, time 16h / d for culture;

[0029] d. Cut off the 1.0-2.0cm axillary buds in step c, insert them into the medium 1 / 2MS+NAA 0.2mg / L, place them at a temperature of 23±1℃, light 2000lx, and time 16h / d to induce rooting;

[0030] e. The seedlings with a height of 9cm in step d, first open the sealing film and smelt the seedlings in the greenhouse for 3d, then wash off the medium of the roots of the ...

Embodiment 3

[0032] a. Pick the plump Yumin thornless safflower seeds, and then sterilize the seeds with 0.1% mercury liters on a clean bench for 20 minutes, 75% alcohol surface disinfection for 1.5 minutes, and rinse with sterile water 6 times;

[0033] b. Put the treated seeds in step a into the germination medium 1 / 2MS, the temperature is 23±1℃, the light is 2000lx, and the time is 16h / d for cultivation;

[0034] c. Cut off the leaves and roots of the 9-day seedling in step b, and insert the 2cm stem into the medium MS+6-BA 1.0mg / L+TDZ 1.0mg / L+PP 333 In 1.0mg / L, placed in temperature 23±1℃, light 2000lx, time 16h / d for culture;

[0035] d. Cut off the 1.0-2.0cm axillary buds in step c, connect them to the medium 1 / 2MS+NAA0.2mg / L+IBA 0.2mg / L, place at a temperature of 23±1℃, light 2000lx, time 16h / Induced rooting under d conditions;

[0036] e. For the seedlings with a height of 10 cm in step d, first open the sealing film and smelt the seedlings in the greenhouse for 3 days, then wash the cul...

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Abstract

The invention relates to a method for tissue culture and rapid propagation of thornless safflower, and provides a seed disinfection method, and an optimal bud proliferation culture medium and an optimal culture medium for inducing buds to root. The method comprises four steps of obtaining aseptic seedlings, proliferating buds, inducing the buds to root, and hardening off and transplanting; and a great number of young plants of the safflower are obtained in short time. The method has the advantages of simpleness, quick propagation, strong stability, short cultivation period, and high rooting rate and easy survival of the thornless safflower, has strong feasibility in the actual production and application, is suitable for industrial cultivation of Xinjiang safflower, and also lays a foundation for researching Yumin thornless safflower polyploidy new species.

Description

Technical field [0001] The invention relates to a method for tissue culture and rapid propagation of thornless safflower. Background technique [0002] Carthamus tinctorius L. is an annual herbaceous plant belonging to the genus Carthamus of the Compositae family, consisting of about 20-25 species. Safflower is widely distributed and cultivated in many countries. my country has a long history of safflower cultivation, and there are records about safflower cultivation and medicinal use in the Han Dynasty. In recent years, the cultivation area of ​​safflower in China has been increasing, mainly for medicinal and oil medicine. China's safflower production areas are mainly concentrated in Xinjiang, Sichuan, Yunnan, Henan and other provinces. Safflower has the effects of promoting blood circulation, clearing blood stasis, healing wounds, spreading rash, etc. The effective medicinal ingredient is safflower pigment, mainly including safflower yellow pigment and safflower red pigment....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 王晓军牛力涛郝秀英
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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