Tissue culture method of corydalis pygmaea (endangered Tibetan medicine)

A tissue culture, Viola dwarfs technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of destruction of the native environment, blank artificial breeding technology of Viola dwarfs, etc. low temperature effect

Inactive Publication Date: 2018-09-14
VEGETABLE RES INST OF TIBET ACADEMY OF AGRI & ANIMAL HUSBANDRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the long-term plundering of wild medicinal materials by using the whole plant as medicine, the original environment has been destroyed, and the wild resources of Corydalis dwarfs have been listed as endangered, and the supply is in short supply.
[0004] The artificial breeding technology of Corydalis dwarf in the existing method is still a blank

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] This embodiment provides a tissue culture method of the endangered Tibetan medicinal material Corydalis dwarfia, the steps comprising:

[0030] (1) Select robust plant buds, wash them quickly with ethanol with a volume concentration of 60%, and then use a concentration of 0.05% HgCl 2 The solution was disinfected for 1 min;

[0031] (2) Rinse the buds treated in step (1) three times quickly with sterile water, then blot the water on the surface of the buds to obtain sterile explants for use;

[0032] (3) Under sterile conditions, inoculate the sterile explants described in step (2) onto the Ms medium, and after cultivating in the dark at 15°C for 10 days, transfer to Ms+6-BA1mg / l+NAA0.01mg On the / l medium, cultivate and induce cluster buds for 30 days under light at 15°C to form cluster buds; the time of the light per day is 18h;

[0033] (4) Transfer the cluster buds into 1 / 2Ms+6-BA0.2mg / l+NAA0.5mg / l, cultivate and take root at 15°C, 1500LX intensity light+natural l...

Embodiment 2

[0036] This embodiment provides a tissue culture method of the endangered Tibetan medicinal material Corydalis dwarfia, the steps comprising:

[0037] (1) Select robust plant buds, first rinse quickly with ethanol with a volume concentration of 80%, and then use a concentration of 0.2% HgCl 2 The solution was disinfected for 3 minutes;

[0038] (2) Rinse the sprouts treated in step (1) rapidly with sterile water 5 times, then blot the water on the surface of the sprouts to obtain sterile explants for use;

[0039] (3) Under sterile conditions, inoculate the sterile explants described in step (2) onto the Ms medium, and after cultivating in the dark at 20°C for 7 days, transfer to Ms+6-BA1mg / l+NAA0.01mg On the / l medium, cultivate and induce cluster buds for 35 days under light at 25°C to form cluster buds; the time of the light per day is 24h;

[0040] (4) Transfer the cluster buds into 1 / 2Ms+6-BA0.2mg / l+NAA0.7mg / l, cultivate and take root at 25°C, 2000LX intensity light+nat...

Embodiment 3

[0043] This embodiment provides a tissue culture method of the endangered Tibetan medicinal material Corydalis dwarfia, the steps comprising:

[0044] (1) Select robust plant buds, wash them quickly with ethanol with a volume concentration of 70%, and then use a concentration of 0.1% HgCl 2 The solution was disinfected for 2 minutes;

[0045] (2) The sprouts treated in step (1) are quickly rinsed 4 times with sterile water, and then the water on the surface of the sprouts is blotted dry to obtain sterile explants for use;

[0046] (3) Under sterile conditions, inoculate the sterile explants described in step (2) onto the Ms medium, and after cultivating in the dark at 18°C ​​for 8 days, transfer to Ms+6-BA1mg / l+NAA0.01mg / l culture medium, at 22 DEG C, cultivate and induce cluster buds under light for 32 days to form cluster buds; the time of light per day is 21h;

[0047] (4) Transfer the cluster buds into 1 / 2Ms+6-BA0.2mg / l+IAA0.5mg / l, cultivate and root at 22°C, 1800LX int...

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Abstract

The invention relates to a tissue culture method of corydalis pygmaea (an endangered Tibetan medicine). The tissue culture method comprises the following steps: sterilizing selected plant buds with ethanol and a HgCl2 solution successively, and then rinsing with sterile water to obtain sterile explants; and carrying out inoculation, shoot bud culture and induction, rooting culture, plantlet hardening and transplanting, and fixed planting on the sterile explants under aseptic conditions, so as to harvest corydalis pygmaea. The tissue culture method provided by the invention fills the blank of tissue culture technique of corydalis pygmaea, is not subject to seasonal restrictions and can be used to produce seedlings all the year round and realize artificial breeding. According to the tissue culture method, the top bud tissue of high-quality robust corydalis pygmaea is sterilized to obtain sterile tissue which is subject to segmentation and differentiation to obtain complete plants, whichhave low cost, can be bred fast, can adapt to high temperature difference, low temperature and strong ultraviolet ray of special environment in a plateau region, have a high seedling speed and good heredity, and can produce seedlings all the year round.

Description

technical field [0001] The invention belongs to the technical field of plant culture, and in particular relates to a tissue culture method of an endangered Tibetan medicinal material Corydalis dwarf. Background technique [0002] Dwarf Corydalis is the plant of Papaveraceae Dwarf Corydalis (dry whole grass, surface brown-red, brown-yellow or dark brown, short stem, capsule oblong) and flat-stalked Corydalis (shrinked into a ball, yellow-brown in appearance To reddish-brown, the leaves are broken and shrunk, the flowers are yellow and thin, and the bracts are oblong-oval) dry whole plant, which has the effect of clearing away heat and reducing inflammation. It can be used to treat alpine plethora, ulcer pain, vasculitis, and enteritis. [0003] Dwarf Corydalis is mainly distributed in the area of ​​4200-5200m above sea level in Tibet, my country. At present, in addition to nearly 100 Tibetan medicine medical institutions and hospital preparation products in Tibet, among the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008Y02P60/40
Inventor 白玛玉珍李淑萍欧珠卫华强巴卓嘎曾钰婷祁驰恒许娟妮
Owner VEGETABLE RES INST OF TIBET ACADEMY OF AGRI & ANIMAL HUSBANDRY SCI
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