Method for tissue culture and rapid propagation of thornless safflower

A technique of tissue culture and thorn safflower, which is applied in botany equipment and methods, soilless cultivation, horticultural methods, etc., can solve problems such as unclear effects, and achieve the effect of simple and practical methods, simple methods, and good heredity

Inactive Publication Date: 2011-06-01
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some studies have been carried out abroad, in actual research, so far, because the tissue culture work of safflower is not as extensive and in-depth as other plants, the factors that affect tissue regeneration in other plants such as genotype, sex and The effects of various factors such as age, tissue source, and physiological state are not very clear in safflower, so further research is needed

Method used

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  • Method for tissue culture and rapid propagation of thornless safflower
  • Method for tissue culture and rapid propagation of thornless safflower
  • Method for tissue culture and rapid propagation of thornless safflower

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] a. Pick plump Yumin thornless safflower seeds, and then sterilize the seeds with 0.1% mercuric chloride on the ultra-clean workbench for 15 minutes, sterilize the surface with 75% alcohol for 1 minute, and rinse with sterile water for 5 times;

[0021] b. Insert the treated seeds in step a into the germination medium 1 / 2MS, culture at a temperature of 23±1°C, light of 2000lx, and time of 16h / d;

[0022] c. Remove the leaves and roots of the seedlings with a seedling age of 7 days in step b, and insert the 1cm stem segment into the medium MS+6-BA 1.0mg / L+TDZ 0.5mg / L+PP 333 0.5mg / L, placed in temperature 23±1℃, light 2000lx, time 16h / d for propagation;

[0023] d. Cut off the 1.0cm axillary buds from the thickened seedlings in step c, insert them into the medium 1 / 2MS+NAA 0.2mg / L, and place them under the conditions of temperature 23±1℃, light 2000lx, and time 16h / d induce rooting;

[0024] e, with the seedlings whose height is 8cm in step d, first open the sealing fil...

Embodiment 2

[0026] a. Pick plump Yumin thornless safflower seeds, and then sterilize the seeds with 0.1% mercuric chloride on the ultra-clean workbench for 17 minutes, sterilize the surface with 75% alcohol for 30 seconds, and rinse with sterile water 4 times;

[0027] b. Insert the treated seeds in step a into the germination medium 1 / 2MS, culture at a temperature of 23±1°C, light of 2000lx, and time of 16h / d;

[0028] c. Remove the leaves and roots of the seedlings with a seedling age of 8 days in step b, and insert the 1.5cm stem segment into the medium MS+6-BA 1.0mg / L+TDZ 0.3mg / L+PP 333 2.0mg / L, cultured at a temperature of 23±1°C, light of 2000lx, and a time of 16h / d;

[0029] d. Cut off the 1.0-2.0cm axillary buds in step c, insert them into the medium 1 / 2MS+NAA0.2mg / L, place them at a temperature of 23±1°C, light 2000lx, and time 16h / d to induce rooting;

[0030] e, with the seedlings whose height is 9cm in step d, first open the sealing film and harden the seedlings in the green...

Embodiment 3

[0032] a. Pick plump Yumin thornless safflower seeds, and then sterilize the seeds with 0.1% mercuric chloride on the ultra-clean workbench for 20 minutes, sterilize the surface with 75% alcohol for 1.5 minutes, and rinse with sterile water for 6 times;

[0033] b. Insert the treated seeds in step a into the germination medium 1 / 2MS, culture at a temperature of 23±1°C, light of 2000lx, and time of 16h / d;

[0034] c. Remove the leaves and roots of the seedlings with a seedling age of 9 days in step b, and insert the 2cm stem segment into the medium MS+6-BA 1.0mg / L+TDZ 1.0mg / L+PP 333 1.0mg / L, cultured at a temperature of 23±1°C, light of 2000lx, and a time of 16h / d;

[0035] d. Cut off the 1.0-2.0cm axillary buds in step c, insert them into the medium 1 / 2MS+NAA0.2mg / L+IBA0.2mg / L, put them at a temperature of 23±1℃, light 2000lx, and time 16h / Induce rooting under d condition;

[0036] e, with the seedlings whose height is 10cm in step d, first open the sealing film and harden...

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Abstract

The invention relates to a method for tissue culture and rapid propagation of thornless safflower, and provides a seed disinfection method, and an optimal bud proliferation culture medium and an optimal culture medium for inducing buds to root. The method comprises four steps of obtaining aseptic seedlings, proliferating buds, inducing the buds to root, and hardening off and transplanting; and a great number of young plants of the safflower are obtained in short time. The method has the advantages of simpleness, quick propagation, strong stability, short cultivation period, and high rooting rate and easy survival of the thornless safflower, has strong feasibility in the actual production and application, is suitable for industrial cultivation of Xinjiang safflower, and also lays a foundation for researching Yumin thornless safflower polyploidy new species.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of thornless safflower. Background technique [0002] Safflower (Carthamus tinctorius L.) is an annual herb belonging to the genus Carthamus in the family Asteraceae, consisting of about 20 to 25 species. Safflower is widely distributed and cultivated in many countries. my country has a long history of safflower cultivation, and there are records about safflower cultivation and medicinal use in the Han Dynasty. In recent years, the cultivated area of ​​safflower in China has been increasing, mainly for both medicinal and oil medicine. China's safflower production areas are mainly concentrated in Xinjiang, Sichuan, Yunnan, Henan and other provinces. Safflower has the functions of activating blood circulation and promoting menstrual flow, removing blood stasis and treating injuries, purifying toxins and clearing rashes, etc. The effective medicinal ingredient is safflower pigme...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 王晓军牛力涛郝秀英
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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