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Pronephrium megacuspe compound, preparation method thereof and application

A compound, the technology of crescent fern, applied in the field of chemistry, achieves the effect of simple preparation method, low preparation cost, and inhibition of cancer cell activity

Inactive Publication Date: 2010-03-17
湖南惠农生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Pronephrium megacuspe compound, preparation method thereof and application
  • Pronephrium megacuspe compound, preparation method thereof and application
  • Pronephrium megacuspe compound, preparation method thereof and application

Examples

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Embodiment 1

[0033] Preparation of Crescentin A, Crescentin B, Crescentin C, Crescentin D, Crescentin E and Crescentin F

[0034] (1) Get 7.5Kg of the whole herb of Crescent fern, dry in the shade, pulverize, extract by cold soaking in 80ml of 95% methanol for 3 times, each time for 3 days, combine the filtrates extracted by cold soaking for 3 times, and concentrate under reduced pressure to obtain The extract is about 800g;

[0035] (2) Suspend the extract in 1 liter of water, extract 3 times with 1.5 liter, 1 liter, and 1 liter of ethyl acetate respectively, combine the extracts for recovery under reduced pressure, and obtain 230 g of ethyl acetate partial extract;

[0036] (3) The ethyl acetate extraction part obtains 4 fractions after 300 mesh silica gel atmospheric pressure column chromatography, column chromatography condition: trichloromethane: methanol=1: 0 to 1: 1; 4 fractions are trichloromethane respectively :methanol=1:0, chloroform:methanol=20:1, chloroform:methanol=10:1, chl...

Embodiment 2

[0051] Embodiment 2 The compound prepared by the present invention has the experiment of biological activity to insect

[0052] The method for measuring the antifeedant activity against the 3rd instar larvae of Ostrinia sativa: the compound prepared by the method of the present invention is dissolved in acetone, and prepared into a mother solution with a concentration of 1000 μg / mL. The mother liquor was prepared with acetone+water to make a drug solution with a concentration of 50 μg / mL, and the final concentration of acetone in the drug was 30%. Using the fresh corn heart leaf method, the diameter of the leaf disk is 1cm, and the leaf disk is soaked with the prepared medicinal solution for 3 seconds, and after the solvent is evaporated, put it into a petri dish lined with wet filter paper. One 3rd instar larva of Ostrinia officinalis starved for 4 hours, each treatment was repeated 10 times, and the leaves were replaced with new pesticides after checking the experimental res...

Embodiment 3

[0060] Embodiment 3 The compound of the present invention has the experiment of cytotoxicity

[0061] Preparation of medicinal solution: Dissolve the test compound prepared by the present invention with a small amount of acetone or dimethyl sulfoxide (DMSO) to make mother liquor, filter and sterilize with a 0.22 μm microporous membrane on the ultra-clean workbench, and use In the medium containing 1% DMSO, the reagents were formulated to the concentration required for the test, the final concentration of acetone was 2.5%, and the final concentration of DMSO was 1%. The medium containing 1% DMSO or 2.5% acetone was used as a control.

[0062] In vitro cytotoxicity test method: MTT method is used. Inoculate SL cells in logarithmic growth phase, human liver cancer cells (SMMC-7721), and human breast cancer cells (MCF-7) into 96-well culture plates, add 100 μL to each well, and culture for 24 hours. Add 100 μL of the corresponding concentration of drug solution to each well, and...

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Abstract

The invention discloses a pronephrium megacuspe compound, a preparation method thereof and application. The invention provides a new chemical structural formula of pronephrium megacuspe compound, wherein the compound is made from plant pronephrium megacuspe with insecticidal activity, bactericidal activity and anticarcinogenic activity. The invention can be applied to preparing insecticide, bactericide and anticarcinogenic medicine. The invention finds out the active compound from the fern for the first time and provides an important technical base for researching mothproof and disease-resistant activities of the fern. The preparation method of the invention is simple and the manufacturing cost is low, so the preparation method is applied to industrial extension.

Description

technical field [0001] The invention belongs to the technical field of chemistry, and in particular relates to a compound prepared from the plant Crescent fern, a preparation method thereof and an application of the compound. Background technique [0002] Due to the same habitat, plants and insects have been living together since ancient times, and the two are interdependent, mutually restricted, and co-evolved. In the long evolutionary process, in order to defend against the harm of insects, plants produce secondary substances in the metabolic process. These secondary metabolites have the functions of repelling food, avoiding, poisoning and inhibiting the growth and development of insects. [0003] At present, botanical pesticides are a hot spot in the research and development of biological pesticides. The theoretical basis of botanical pesticide research is the co-evolution relationship between plants and insects. Due to the close relationship between angiosperms and moder...

Claims

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Application Information

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IPC IPC(8): C07D311/32C07H17/07C07D493/04C07D311/40C07H1/08A01N43/16A61K31/352A61K31/7048A61K31/366A01P7/04A01P3/00A61P35/00
Inventor 徐汉虹黄素青魏孝义田永清
Owner 湖南惠农生物工程有限公司
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