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Fragrant solomonseal rhizome isoflavanone extract as well as preparation method and application thereof

A technology of high isoflavones and extracts, which is applied in the field of Polygonatum Polygonatum Dihydrogenated High Isoflavones Extract and its preparation and application, to achieve clear action mechanism, high content, and improved curative effect

Active Publication Date: 2010-03-10
吉林亚泰永安堂药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the prevention and / or treatment of diabetic nephropathy by Polygonatum dihydrogenoisoflavones

Method used

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  • Fragrant solomonseal rhizome isoflavanone extract as well as preparation method and application thereof
  • Fragrant solomonseal rhizome isoflavanone extract as well as preparation method and application thereof
  • Fragrant solomonseal rhizome isoflavanone extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Determination of 3-(4'-Hydroxyphenyl)-5,7-dihydroxy-6-methyl-8-oxomethyl-phenylpropanedihydropyran-4 in Polygonatum Polygonatum Dihydrohomoisoflavone Extract by HPLC -ketone, 3-(4'-hydroxyphenyl)-5,7-dihydroxy-6,8-dimethyl-phenylpropanedihydropyran-4-one and 3-(4'-methoxyphenyl )-5,7-dihydroxy-6-methyl-8-oxymethyl-phenylpropanedihydropyran-4-one content method

[0033] Chromatographic conditions and system suitability test

[0034] Octadecylsilane bonded silica gel is used as filler; water is used as mobile phase A, methanol is used as mobile phase B, linear gradient elution: 0min (A 60%, B 40%)→10min (A 40%, B 60%)→30min(A 30%, B 70%)→40min(A 0%, B 100%); flow rate: 1.0mL min -1 ; The detection wavelength is 254nm. The number of theoretical plates is calculated based on the peak of 3-(4'-hydroxyphenyl)-5,7-dihydroxy-6,8-dimethyl-phenylpropanedihydropyran-4-one, not less than 4000.

[0035] Solution preparation

[0036] Preparation of reference solution

[0037] A...

Embodiment 2

[0043] Take 100Kg of Polygonatum rhizome powder, add 9 times the amount of ethanol to reflux and extract 3 times, each time for 2 hours, filter, combine the extracts, and recover the extract under reduced pressure. Take the extract and dissolve it in water, add it to HPD100 macroporous adsorption resin column, elute with water until the eluent is nearly colorless, discard the eluent, and elute with 50% ethanol until the eluent is nearly colorless, discard the eluent Remove the liquid, elute with ethanol until the eluate is nearly colorless, collect the eluate, and recover under reduced pressure to obtain 642 g of polygonatum dihydrohomoisoflavone extract. The method of Example 1 is used to measure 3-(4'-hydroxyphenyl)-5,7-dihydroxy-6-methyl-8-oxomethyl-phenylpropanedihydro in Polygonatum Polygonatum Dihydrogen-Isoflavone Extract Pyran-4-one, 3-(4'-hydroxyphenyl)-5,7-dihydroxy-6,8-dimethyl-phenylpropanedihydropyran-4-one and 3-(4'- The sum of the contents of methoxyphenyl)-5,7...

Embodiment 3

[0045] Take 100Kg of Polygonatum rhizome powder, add 12 times the amount of 90% ethanol to reflux and extract for 3 times, each time for 2 hours, filter, combine the extracts, and recover under reduced pressure to obtain the extract. Take the extract and dissolve it in water, add it to HPD200 macroporous adsorption resin column, elute with water until the eluent is nearly colorless, discard the eluent, and elute with 40% ethanol until the eluent is nearly colorless, discard the eluent Remove the liquid, elute with 90% ethanol until the eluate is nearly colorless, collect the eluate, and recover under reduced pressure to obtain 895 g of Polygonatum dihydrogenum isoflavone extract. The method of Example 1 is used to measure 3-(4'-hydroxyphenyl)-5,7-dihydroxy-6-methyl-8-oxomethyl-phenylpropanedihydro in Polygonatum Polygonatum Dihydrogen-Isoflavone Extract Pyran-4-one, 3-(4'-hydroxyphenyl)-5,7-dihydroxy-6,8-dimethyl-phenylpropanedihydropyran-4-one and 3-(4'- The sum of the conte...

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Abstract

The invention provides a fragrant solomonseal rhizome isoflavanone extract and a preparation method thereof. The fragrant solomonseal rhizome isoflavanone extract contains 3-(4'- hydroxyphenyl)-5,7-dyhydroxy-6-methyl-8-O-methylation-styrene acrylic dihydropyrane-4-ketone, 3-(4'-hydroxyphenyl)-5,7-dyhydroxy-6,8-dimethyl-styrene acrylic dihydropyrane-4-ketone and 3-(4'-methoxyphenyl)-5,7-dyhydroxy-6-methyl-8-O-methylation-styrene acrylic dihydropyrane-4-ketone, and the content sum of all components is larger than or equal to 15 percent. The invention also discloses an application of the fragrantsolomonseal rhizome isoflavanone extract in preparing a product for preventing and / or treating diabetic nephropathy and an application of a fragrant solomonseal rhizome isoflavanone compound containing the 3-(4'- hydroxyphenyl)-5,7-dyhydroxy-6-methyl-8-O-methylation-styrene acrylic dihydropyrane-4-ketone, the 3-(4'-hydroxyphenyl)-5,7-dyhydroxy-6,8-dimethyl-styrene acrylic dihydropyrane-4-ketone and the 3-(4'-methoxyphenyl)-5,7-dyhydroxy-6-methyl-8-O-methylation-styrene acrylic dihydropyrane-4-ketone in preparing an inhibitor product as a protein nonenzyme glycolysis end product.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to Polygonatum Polygonatum Dihydrogenated High Isoflavones Extract and a preparation method thereof, and also relates to the use of Polygonatum Polygonatum Dihydrogenated High Isoflavones Extract in preparing products for preventing and / or treating diabetic nephropathy, Polygonatum Polygonatum Dihydrogenate High isoflavone compound 3-(4'-hydroxyphenyl)-5,7-dihydroxy-6-methyl-8-oxomethyl-phenylpropanedihydropyran-4-one, 3-(4' -hydroxyphenyl)-5,7-dihydroxy-6,8-dimethyl-phenylpropanedihydropyran-4-one and 3-(4'-methoxyphenyl)-5,7-dihydroxy -Application of 6-methyl-8-oxymethyl-phenylpropanedihydropyran-4-one in the preparation of protein non-enzymatic glycation end product inhibitor products. Background technique [0002] Diabetic nephropathy is one of the common chronic microvascular complications of diabetes. It usually refers to glomerular basement membrane thickening, mesangial expansion, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P13/12A61P3/10
Inventor 王威师海波
Owner 吉林亚泰永安堂药业有限公司
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