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Method for extracting protein peptide from membranes of fowl eggshells

A protein peptide and eggshell technology, which is applied in the field of enzymatic hydrolysis of antioxidant active peptides, and the extraction of protein peptides from eggshell membranes, to achieve the effect of strong operability and simple process.

Inactive Publication Date: 2010-02-17
黄继红
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no patent applications and related reports on the use of eggshells to enzymatically hydrolyze small peptides with antioxidant activity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] a. Take 50g of eggshells and ultrafinely pulverize them to 300-600 mesh;

[0017] b. Use organic acid as a solvent to adjust the pH value to 3.5-4.5;

[0018] c. Prepare eggshell suspension, with a solid content of 10%;

[0019] d. Separate the non-protein components in the eggshell;

[0020] e. Put the extracted eggshell protein into a container with a stirrer, adjust the eggshell suspension with deionized water,

[0021] e.1 The alkali used is ammonia water or quaternary ammonium salt

[0022] e.2 Adjust the pH value to 5-9

[0023] e.3 Solid content of eggshell suspension 10%

[0024] e.4 Add 0.15g of enzyme preparation

[0025] e.5 Heat at 20°C-60°C and stir thoroughly for 2-3 hours;

[0026] f. Add 0.15 g of peptidase, keep warm at 20°C-60°C, stir thoroughly for 9 hours, then centrifuge to remove the residue to obtain small peptide nutrient solution;

[0027] g. Concentrate, evaporate and dry at 60°C to obtain small peptide products.

Embodiment 2

[0029] a. Eggshell 2Kg is superfinely pulverized to 600 mesh;

[0030] b. Use organic acid as a solvent to adjust the pH value to 3.5-4.5;

[0031] c. prepare eggshell suspension, solid content 8%;

[0032] d. Separate the non-protein components in the eggshell;

[0033] e. Put the separated eggshell protein into a container with a stirrer, adjust the eggshell suspension with deionized water,

[0034] e.1 The alkali used is ammonia water or quaternary ammonium salt

[0035] e.2 Adjust the pH value to 5-9

[0036] e.3 Solid content of eggshell suspension 6%

[0037] e.4 Add 6g of enzyme preparation

[0038] e.5 Heat at 20-60°C and stir thoroughly for 2-4 hours;

[0039] f. Add 6g of peptidase, keep warm at 20°C-60°C, stir thoroughly for 10 hours, then centrifuge to remove the residue, and obtain the small peptide nutrient solution;

[0040] g. Concentrate, evaporate and dry at 60°C to obtain small peptide products.

[0041] Determination of small peptide content: GB / T66...

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PUM

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Abstract

The invention discloses a method for extracting protein peptide from the membranes of fowl eggshells. The technical scheme of the method comprises the following steps: pulverizing eggshells in a superfine way; regulating the pH value to 3-5 by using organic acid as a solvent and preparing eggshell suspension; separating out nonprotein components in the eggshells; regulating eggshell protein afterbeing separated by deionized water, regulating the pH value to 5-9 by alkali, adding an enzyme preparation, heating and stirring; then adding peptidase, stirring, afterwards separating by centrifugation and removing material residues so as to obtain a small-peptide nutrient solution; and carrying out concentrating evaporation at 50-60 DEG C, drying and obtaining a small-peptide product. The invention has simple technique and strong operability, and the content of small peptide prepared by the invention is as high as more than 50%.

Description

technical field [0001] The invention relates to an enzymatic hydrolysis preparation method of antioxidant active peptides, in particular to a method for extracting protein peptides from poultry eggshell membranes, belonging to the field of food and medicine. Background technique [0002] my country is a big consumer of poultry eggs, and its food processing factories, pastry factories, hatcheries, hotels, corporate canteens and other units discard about 4 million tons of eggshells every year, which not only pollutes the environment, but also wastes resources. Because discarded eggshells will quickly rot and deteriorate, breeding bacteria, especially in summer, there are swarms of mosquitoes and flies, and the smell is unpleasant. In fact, eggshell is a kind of complete and highly combined biological calcium, composed of inorganic matter and organic protein, containing 2.8% organic matter, and the organic matrix mainly includes glycoprotein and glycopeptide. The eggshell memb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 黄继红杨玉萍
Owner 黄继红
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