Method for separating and purifying amikacin

Abstract

The invention discloses a method for separating and purifying amikacin, which carries out continuous chromatographic resolution on amikacin material by using weak-acid cation resin and employing a continuous chromatographic resolution system technique. The method comprises the following steps: the material solution divides 30 separating units in the whole procedure into four regions comprising anadsorption region, a washing region, a separating region and a regenerating region through a continuous chromatographic resolution system, so as to replace the prior fixed-bed separation plant; and the original steps of absorption, washing, resolving, regenerating and the like in the fixed bed in the whole procedure are integrated into the disc-transmission type reverse-flow continuous chromatographic resolution system, so that the amikacin is effectively separated and purified, the production cost is reduced, the production technique is simplified, the production period is shortened, the pollution is reduced and the total yield is increased.

Description

technical field [0001] The invention relates to a method for separating and purifying amikacin, in particular to a method for separating and purifying amikacin by adopting continuous chromatographic separation system technology. Background technique [0002] Amikacin is an aminoglycoside antibiotic used to treat a variety of bacterial infections. Amikacin relies on the combination of bacterial 30S subunit to block the synthesis of bacterial protein to play an antibacterial effect. It is often used to treat severe multidrug-resistant Gram-negative bacterial hospital-acquired infections, such as Pseudomonas aeruginosa, Acinetobacter and Enterobacter, etc. [0003] At present, the production method of amikacin generally adopts kanacin as raw material through chemical reaction, and there are mainly the following components in the final reaction mixture: AHBA, KNA, K6, K29, K8, K11. The contents of K6 and K11 in the reaction are relatively low; while K8 and K29 ​​are isomers wi...

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Problems solved by technology

[0004] 1) The production cycle is very long. Due to the large amount of resin, the utilization rate per unit of resin is very low, and the fixed bed has a large amount of one-time investment and occupies a very large area;

[0005] 2) The amount of regeneration reagent is large (including water), and the environmental pollution is relatively serious, especially the ammonia-containing wastewater, which has a very high pressure on environmental protection;

[0006] 3) The separation effect of K8 and K29 ​​is poor. In the resin analysis collection solution, the mixed solution of K8 and K29 ​​accounts for a large proportion; the purity of the K8 collection solution is low, so as to reduce the yield of the subsequent section;

[0007] 4) The yield of the whole resin extraction section is low

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