Brucella abortus recombinant strain and application thereof

Abstract

The invention discloses a brucella abortus recombinant strain and the application thereof, and the brucella abortus is obtained by inactivating glycosyltransferase SLT subunit protein gene in strain 2308. Compared with the original strain, the strain obtained in the invention has little strain toxicity, good immunity protecting effect and potential differential diagnosis mark, and can be used for research and development of attenuated vaccine. The invention is beneficial to the reformation of brucellosis vaccine, the research and the preparation of differential diagnosis reagent and the development of the brucellosis vaccine and the differential diagnosis reagent matched with the vaccine, and has very important significance on promoting the elimination of brucellosis of the animals all over the world.

Description

technical field [0001] The invention relates to a recombinant bacterium of Brucella abortus and its application, in particular to a recombinant bacterium of an inactivated glycosyltransferase SLT subunit protein gene and its application. Background technique [0002] Brucellosis is a zoonotic infectious disease, second only to foot-and-mouth disease among large animal infectious diseases, and has important political and economic influences. The epidemic situation in countries around the world is very serious. For a long time, the global strategy for the prevention and control of brucellosis has been mainly quarantine-culling (in developed countries) or immunization (in developing countries). The former uses quarantine and culling of sick cattle, sheep, pigs and other animals as a measure, which costs a lot of money , Difficult to operate; the latter can prevent the spread of the disease in the animal population through immunization and auxiliary culling measures, but it is d...

AI Technical Summary

Problems solved by technology

For a long time, the global strategy for the prevention and control of brucellosis has been mainly quarantine-culling (in developed countries) or immunization (in developing countries). The former uses quarantine and culling of sick cattle, sheep, pigs and other animals as a measure, which costs a lot of money , Difficult to operate; the latter can prevent the spread of the disease in the animal population through immunization and auxiliary culling measures, but it is difficult to distinguish between immunized animals and clinically diseased animals

Although rough strain vaccines allow for differential diagnosis, allowing the distinction of immunized animals from clinically infected animals, the protective potency of this type of vaccine is significantly lower

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