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Blood lipid-lowering active fractions of Chinese date, extraction method thereof and application thereof

A technology of effective parts and extraction method, applied in the application field of preparing blood lipid-lowering and antioxidant drugs, can solve the problems of side effects, muscle toxicity, single action target, etc., and achieves lower blood lipid level, simple process and good social value. and economic value

Inactive Publication Date: 2010-01-20
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although Western medicine has a certain effect on regulating blood lipids, it has side effects of varying degrees, and the target is single.
Western medicine statins are currently recognized as the most effective and widely used drugs for lipid-lowering, but they have side effects such as liver and muscle toxicity

Method used

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  • Blood lipid-lowering active fractions of Chinese date, extraction method thereof and application thereof
  • Blood lipid-lowering active fractions of Chinese date, extraction method thereof and application thereof
  • Blood lipid-lowering active fractions of Chinese date, extraction method thereof and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The blood lipid-lowering effective part of the jujube of the present invention is the jujube polysaccharide obtained by soaking the pitted jujube in ethanol, air-drying the residue, boiling three times with water, and combining the concentrate.

Embodiment 2

[0016] The method for extracting the effective part of jujube for lowering blood fat of the present invention specifically comprises the following steps: repeatedly cold-soaking the pitted jujube with 90% edible ethanol at a volume ratio of 5:1 for 3 times, each time for 7 days, filtering, and air-drying the filter residue, The air-dried filter residue was boiled in water for 3 times, each time for 15 minutes, the obtained extracts were combined and concentrated, and dried to obtain a dry product; the obtained dry product was dissolved in water, deproteinized with 30% trichloroacetic acid, left standing, centrifuged, and then dried The clear liquid is adjusted to pH = 7 with lye, concentrated under reduced pressure, and the concentrated liquid is dialyzed against water. The concentrated liquid after dialyzing is added with absolute ethanol to make the alcohol content 70%, let stand, centrifuge, collect the precipitate, and obtain the large Jujube polysaccharide.

Embodiment 3

[0018] In this example, the anti-oxidation test in vitro and the anti-oxidation test in vivo for blood lipid-lowering and anti-oxidation were carried out on the effective part of jujube-jujube polysaccharide, the details are as follows:

[0019] Antioxidant test in vitro:

[0020] The polysaccharide parts of jujube were studied as follows:

[0021] Materials and Reagents:

[0022] Jujube polysaccharides were prepared by the laboratory. Diphenyl bitter hydrazine free radical DPPH (Tokyo Chemical Industry Co., Ltd., Japan); Fe 3+ -Tripyridine triazine TPTZ (Acros organics); Trolox (Aldrich); ABTS [2,2'-hydrazine-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] (Fluka); BHT (Acros organics); methanol, sodium acetate, FeCl 3 ·6H 2 O, glacial acetic acid, and potassium peroxodisulfate were all analytically pure.

[0023] Main instruments:

[0024] Multiskan MK3 microplate reader (US thermo Electron company), UV-2000 ultraviolet-visible spectrophotometer (Unico Shang...

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Abstract

The invention discloses blood lipid-lowering active fractions of Chinese date, an extraction method thereof and application thereof. The active fractions are Chinese date polysaccharides which are obtain by extracting core removal Chinese date. The extraction method comprises the following steps: airing the core removal Chinese date which is soaked in 90 percent eatable alcohol, decocting the aired core removal Chinese date for 15 minutes with water, combining concentrated solution, and removing protein by adopting a trichloroacetic acid method to obtain the Chinese date polysaccharides. The anti-oxidation screening in vitro discovers that the Chinese date polysaccharides at the blood lipid-lowering active fractions of the Chinese date have anti-oxidation activity, and also have good blood lipid-lowering function in vivo and in vitro. In addition, the extraction method for the Chinese date polysaccharides has the advantages of simple process and short operating period, greatly reduces the production cost, and has good social value and economic value and quite broad application prospect.

Description

technical field [0001] The present invention relates to extracting and preparing blood lipid-lowering effective parts from jujube, and also relates to the extraction method of the effective parts and the application of the effective parts in the preparation of blood fat-lowering and antioxidant drugs. Background technique [0002] Jujube is the ripe fruit of the rhamnaceae plant jujube (ziziphus Jujube Mill), which is warm in nature, sweet in taste and non-toxic. It is mainly used to treat evil qi in trusted subordinates, tranquilize the middle, nourish the temper, calm the stomach qi, unblock the nine orifices, help the twelve meridians, replenish less qi, less body fluid, insufficiency in the body, severe shock and heavy limbs, and hundreds of medicines, long-term use to lighten the body and prolong the body. Jujube is delicious and sweet, and its sugar content ranks first in all kinds of fruits. The sugar content of fresh dates is more than 20%, and the sugar content of d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/725A61P3/06A61P39/06A61K131/00
Inventor 康文艺姬志强
Owner HENAN UNIVERSITY
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