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Inorganic phosphorus (phosphate radical) diagnosis/determination kit and method for determining inorganic phosphorus (phosphate radical) concentration

A technology of phosphate radical and inorganic phosphorus, which is applied in the field of determination of inorganic phosphorus concentration and inorganic phosphorus diagnosis/measurement kit, which can solve the problem of high results

Inactive Publication Date: 2010-01-06
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, jaundice, hemolysis, and fat-turbid serum have light absorption at 340nm, and a blank specimen must be prepared, otherwise the result will be high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The inorganic phosphorus (phosphate radical) diagnosis / measurement reagent of the present embodiment is a single reagent, comprising:

[0047] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0048] Stabilizer 500mmol / L

[0049] Coenzyme 3mmol / L

[0050] 5' Nucleotidase 10000U / L

[0051] Acetyl-CoA synthetase 12000U / L

[0052] Pyruvate dehydrogenase 12000U / L

[0053] Adenosine 6mmol / L

[0054] Diphosphoric acid 12mmol / L

[0055] Acetyl-CoA 8mmol / L

[0056] Pyruvate 16mmol / L

[0057] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0058]Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured inorganic phosphorus (phosphate) sample to the reagent is 1 / 25, the ...

Embodiment 2

[0061] The inorganic phosphorus (phosphate radical) diagnosis / measurement reagent of the present embodiment is double reagent, comprises:

[0062] Reagent 1

[0063] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0064] Stabilizer 50mmol / L

[0065] Coenzyme 3mmol / L

[0066] Adenosine 6mmol / L

[0067] Diphosphoric acid 12mmol / L

[0068] Acetyl-CoA 8mmol / L

[0069] Pyruvate 16mmol / L

[0070] Reagent 2

[0071] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0072] Stabilizer 50mmol / L

[0073] 5' Nucleotidase 10000U / L

[0074] Acetyl-CoA synthetase 12000U / L

[0075] Pyruvate dehydrogenase 12000U / L

[0076] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0077] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, t...

Embodiment 3

[0080] The inorganic phosphorus (phosphate radical) diagnosis / measurement reagent of the present embodiment is three reagents, comprises:

[0081] Reagent 1

[0082] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0083] Stabilizer 50mmol / L

[0084] Coenzyme 3mmol / L

[0085] Adenosine 6mmol / L

[0086] Diphosphoric acid 12mmol / L

[0087] Acetyl-CoA 8mmol / L

[0088] Pyruvate 16mmol / L

[0089] Reagent 2

[0090] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0091] Stabilizer 500mmol / L

[0092] Acetyl-CoA synthetase 12000U / L

[0093] Pyruvate dehydrogenase 12000U / L

[0094] Reagent 3

[0095] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0096] Stabilizer 500mmol / L

[0097] 5' Nucleotidase 10000U / L

[0098] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0099] When measuring the concentration of inorganic p...

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PUM

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Abstract

The invention relates to an inorganic phosphorus (phosphate radical) diagnosis / determination kit using the technology of an enzyme colorimetric method and an ELISA method, a method for determining inorganic phosphorus (phosphate radical) concentration and composition and components of a reagent, and belongs to the technical field of test and determination of medical science, food, and environment. The kit comprises the following main components: buffer solution, coenzyme, adenoside, bisphosphate, acetyl coenzyme A, pyruvic acid, 5'nucleotidase, acetyl-coA-synthetase, pyruvate dehydrogenase and a stabilizer. The method comprises the following steps: mixing a sample and the reagent according to a certain volume ratio to perform a series of enzymic reaction, and then placing reactants under a UV / visible light analyzer to test the ascending speed of absorbance at the position where the dominant wave length is 340nm to determine the inorganic phosphorus (phosphate radical) concentration.

Description

technical field [0001] The invention relates to an inorganic phosphorus (phosphate) diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring the concentration of the inorganic phosphorus (phosphate), which belongs to the technical field of medicine / food / environmental inspection and measurement. Background technique [0002] 87% of the phosphorus in the human body exists in the bones. The phosphorus in the blood and the phosphorus in the bones maintain a dynamic balance. There is a certain relationship between the concentration of calcium and phosphorus in the blood. When the blood calcium increases in normal people, the blood phosphorus decreases, and vice versa. Of course. The product of [calcium] and [phosphorus] concentrations in plasma remains within a certain range; When the product of the two is greater than 40, calcium and phosphorus are deposited in the bone tissue in the form of bone salt, and when the product is greater...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/25G01N33/84C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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