Method for efficiently saccharifying xylose residue or furfural residue
A technology of furfural residue sugar and xylose residue, which is applied in the field of high-efficiency saccharification of xylose residue or furfural residue, which can solve the problems of affecting the use effect of cellulase, difficulty in meeting the fermentation process, and substrate concentration restrictions, so as to promote industrial recovery and reuse, beneficial to environmental protection, and the effect of reducing the amount of cellulase
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Embodiment 1
[0020] Add xylose slag (solid residue after dilute acid hydrolysis of corncob) with a water content of 70% into the steam explosion machine, open the steam valve and quickly heat up to 200°C, the heating time is 1 minute, and after 15 minutes of heat preservation at 200°C, Close the steam valve, open the steam explosion quick-opening valve of the steam explosion machine to release the material instantly. Collect the material after steam explosion treatment through centrifugation, the gained solid phase adds the water of same weight and adjusts pH to 4.8 with acid, regulates temperature to 50 ℃, then adds cellulase, and enzyme amount is 30FPIU / gram substrate (with dry weight), the stirring speed was 100rpm / min, and the enzymolysis time was controlled at 24 hours. Samples were taken every 12 hours, the total sugar concentration in the enzymolysis solution was measured by DNS method, and the glucose and xylose concentrations in the enzymolysis solution were measured by high perfo...
Embodiment 2
[0022] Add xylose slag with a water content of 60% (the solid residue after dilute acid hydrolysis of corncobs) into the steam explosion machine, open the steam valve and quickly raise the temperature to 190°C, the heating time is 2 minutes, and after 10 minutes of heat preservation, close the steam valve , Open the steam explosion quick-opening valve of the steam explosion machine to release the material instantly. The material after steam explosion treatment is collected and centrifuged, the obtained solid phase is added to the container, and the same weight of water is added at the same time, the pH is adjusted to 4.6 with acid, the temperature is adjusted to 46 °C, and then cellulase is added. The amount of enzyme used is 20 FPIU / Gram substrate (in dry weight), the stirring speed is 100rpm / min, and the enzymolysis time is controlled at 72 hours. Samples were taken every 12 hours, the total sugar concentration in the enzymolysis solution was measured by DNS method, and the...
Embodiment 3
[0024] Add xylose slag with a water content of 80% into the steam explosion machine, open the steam valve and quickly raise the temperature to 220°C, the heating time is 2 minutes, after 2 minutes of heat preservation, close the steam valve, open the steam explosion quick opening valve of the steam explosion machine The material is released instantly. The material after steam explosion treatment is collected and centrifuged, the obtained solid phase is added to the container, and the same weight of water is added at the same time, the pH is adjusted to 5.0 with acid, the temperature is adjusted to 48 °C, and then cellulase is added. The amount of enzyme used is 25 FPIU / Gram substrate (in dry weight), the stirring speed is 100rpm / min, and the enzymolysis time is controlled at 64 hours. Samples were taken every 12 hours, the total sugar concentration in the enzymolysis solution was measured by DNS method, and the glucose and xylose concentrations in the enzymolysis solution wer...
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