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Application of mangosteen nutshell total extract in preparing inflammation-resisting pain-relieving medicine

An anti-inflammatory and analgesic extract technology, applied in the application field of the total extract of mangosteen husk in the preparation of anti-inflammatory and analgesic drugs, to achieve good analgesic and anti-inflammatory, good gastrointestinal tract protection, and good anti-inflammatory effects Effect

Inactive Publication Date: 2012-05-09
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ketone compounds have a wide range of physiological activities, are good nerve inhibitors and cardiovascular stimulants, and have the functions of detoxification, liver protection, anticonvulsion, and inhibition of monoamine oxidase. Biological activities such as anti-tumor and immunity, there are no related reports on the use of mangosteen husk extracts for anti-inflammatory and analgesic drugs

Method used

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  • Application of mangosteen nutshell total extract in preparing inflammation-resisting pain-relieving medicine
  • Application of mangosteen nutshell total extract in preparing inflammation-resisting pain-relieving medicine
  • Application of mangosteen nutshell total extract in preparing inflammation-resisting pain-relieving medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation of embodiment 1 mangosteen husk total extract

[0034] Wash the mangosteen husk, place it in a constant temperature drying oven, dry it at 60°C until it reaches a constant weight, and weigh it to obtain 4.37kg; break the dried mangosteen husk into pieces, and add 6.4L of 95% by volume ethanol for extraction. One week later, the extract was filtered, the filtrate was taken, and concentrated; the concentrate was vacuum-dried for 24 hours to prepare a vacuum-dried extract, which was the total extract of mangosteen husk (CEM for short).

Embodiment 2

[0035] The acute toxicity experiment of embodiment 2CEM

[0036] In this example, the acute toxicity test was carried out on the total extract of mangosteen husk (abbreviated as CEM) prepared in Example 1.

[0037] 1. Maximum tolerated dose test (MTD)

[0038] 20 mice, half male and half male, were divided into two groups, fasted for 12 hours before the experiment, and given two different concentrations of mangosteen crude extract solutions (2gCEM / ml concentration group and 5.8gCEM / ml concentration group) each time i.g. 5.8g CEM / ml and 2g crude drug / ml. The solution was 0.4ml per gavage, once every 2 hours, five times in a row, then observed for 7 days and recorded the animal's food intake, mental state, activity and death, during which the mice were free to eat and drink. Once the mice were found to be dead, the gavage every 2 hours was stopped immediately, and the remaining mice were observed for 7 days. The total gavage dose before death was taken as the maximum tolerate...

Embodiment 3

[0050] Embodiment 3 writhing method measures the analgesic activity of CEM

[0051] In this example, the analgesic activity of the CEM prepared in Example 1 was measured by the writhing method using the routine operations of those skilled in the art.

[0052] Select 50 mice, half male and half female, and divide them into normal saline group, indomethacin group, CEM low dose group (20g / kg), CEM middle dose group (40g / kg) and CEM high dose group (80g / kg ), a total of five groups, 10 rats / group, in which the normal saline group was the control group, and the indomethacin group was the positive control group.

[0053] Fasting for 12 hours before the experiment, 1 hour after the administration, intraperitoneally inject 0.6% acetic acid, 0.2ml / mouse, and count the times of writhing of the mice within 20 minutes. A writhing response was defined as one extension of mouse abdomen and hind limbs due to stimulation. The experimental results are reflected by the percentage of inhibitio...

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Abstract

The invention discloses an application of a mangosteen nutshell total extract in preparing inflammation-resisting pain-relieving medicine. Ethanol with the volume of 95% is adopted to leach a mangosteen nutshell, a leaching solution is collected, the mangosteen nutshell total extract is obtained by filtration, concentration and drying, and an experiment for the acute toxicity, the pain-relieving activity, the inflammation-resisting activity and the function of removing hydroxy free radicals is carried out on the mangosteen nutshell total extract; and a result shows that the extract has very good pain-relieving and inflammation-resisting activities and can be used for preparing the inflammation-resisting pain-relieving medicine. Compared with the traditional non-steroid inflammation-diminishing pain-relieving medicine, the toxic or side reaction of the invention on the stomach and the bowel is obviously lowered; and the invention also has a certain removing function on the hydroxy freeradicals and has the functions of resisting oxidation and protecting nerves.

Description

technical field [0001] The invention relates to the fields of natural product chemistry and medicinal chemistry, in particular to the application of active ingredients in the mangosteen husk, and in particular to the application of the total extract of the mangosteen husk in the preparation of anti-inflammatory and analgesic drugs. Background technique [0002] Pain and inflammatory response are warning signals, indicating that the body has already suffered tissue damage or is about to suffer damage, and it is a defense response of the body's self-protection. If the pain and inflammation persist for a long time, it will constitute an unbearable mental torture to the body, seriously affect study, work, diet and sleep, and eventually cause economic and social problems that cannot be ignored due to the reduced quality of life. [0003] Pain and inflammation are a chain reaction, the two are mutually causal and affect each other, resulting in anti-inflammatory and analgesic drug...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P29/00A61K131/00
Inventor 项辉胡雯崔继红
Owner SUN YAT SEN UNIV
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