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Method for preparing biological preparation for efficiently poisoning plant nematodes

A technology of biological preparations and plants, applied in the direction of botany equipment and methods, biocides, nematicides, etc., to achieve significant poisoning effect, simple method, and environment-friendly effect

Inactive Publication Date: 2009-11-25
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far no patents and reports about the nematicidal activity of Bacillus amyloliquefaciens

Method used

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  • Method for preparing biological preparation for efficiently poisoning plant nematodes
  • Method for preparing biological preparation for efficiently poisoning plant nematodes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. Preparation of line-killing biological preparations

[0018] Inoculate Bacillus amyloliquefaciens on beef extract peptone slant medium, culture at 25-37°C for 24 hours to obtain test tube species, and make 10 test tube species 9 Cfu / mL seed liquid, inoculated into 250mL Erlenmeyer flask liquid medium, the liquid medium formula is: 2-10% beef extract, 1-10% peptone powder, 0.1-3% K 2 HPO 4 , 0.1~1% MgSO 4 , 1-5% NaCl, 100mL of fresh liquid medium in each bottle, 1mL of inoculum, shaker culture at pH 6.5-8, 25-37°C for 2-15d, and the rotation speed is 120rpm, the fermentation culture Centrifuge at 4000rpm for 20min, and the supernatant is a biological agent with high toxic and nematicidal activity. The control is the supernatant of the sterile liquid medium centrifuged at 4000rpm.

[0019] 2. Cultivation and preparation of test nematodes

[0020] First inoculate Botrytis cinerea (Botrytis cinerca) on the PDA plate, culture at 25°C and under the condition of no lig...

Embodiment 2

[0027] 1. Preparation of nematocide biological preparation: same as part "1" in Example 1

[0028] 2. Preparation of test nematodes: Radopholus similis and Aphelenchoides besseyi were separated from host plants by funnel method, and the preparation of the rest of the nematodes was the same as in the "2" part of Example 1.

[0029] 3. Drug efficacy assay method: same as "3" part in Example 1

[0030] 4. Test results

[0031] Result (table 2) shows: this biological preparation all has nematicidal activity to the multiple nematodes listed in table 2, to banana perforator nematode, rice dry point nematode, pine wood nematode, pseudo pine wood nematode, large nucleus smooth edge nematode The nematode nematode killing rate reached 100% within 24 hours, and the control nematode killing rate was 0%, indicating that it has high nematicidal activity and a wide nematicidal spectrum, and has good application and development prospects.

[0032] Table 2 Comparison of nematicidal activity ...

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Abstract

The invention relates to a method for preparing a biological preparation for efficiently poisoning plant nematodes, which belongs to the technical field of microbial pesticides. A strain that the invention relates to is Bacillus amyloliquefaciens. The method for preparing the biological preparation comprises the following steps that: a Bacillus amyloliquefaciens test tube seedling is inoculated to a liquid culture medium (the formula is 2 to 10 percent of beef extract, 1 to 10 percent of peptone powder, 0.1 to 3 percent of K2HPO4, 0.1 to 1 percent of MgSO4, and 1 to 5 percent of NaCl); and the volume ratio of loading capacity is 2 / 5, a shaking table performs shaking culture under the conditions that the pH value is between 6.5 and 8 and the temperature is between 25 and 37 DEG C, the rotating speed is 120 rpm, cultured zymotic fluid is centrifugated for 20 min (4,000 rpm) after 2 to 15 days, and a supernatant fluid is the biological preparation with high nematicidal activity. Through the determination of pesticide effectiveness, the product has 100 percent of poisoning rate on the plant nematodes such as Bursaphelenchusxylophilus, Radopholus similis, Aphelenchoides besseyi and the like, and has broad development and application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and specifically refers to a method for preparing a highly effective biological agent for poisoning and killing plant parasitic nematodes by using Bacillus amyloliquefaciens. Background technique [0002] Plant-parasitic nematodes refer to nematodes that parasitize plants and have a unique feeding organ—a stylet. There are more than 200 genera and more than 5,000 species that have been recorded so far. Almost all plants are parasitized and damaged by nematodes. According to the statistics of the Food and Agriculture Organization of the United Nations, the annual losses caused by nematodes in the world exceed 100 billion US dollars. In my country, it is reported that there are nearly a hundred kinds of nematode diseases that seriously threaten agricultural, forestry, economic and other crops. For example, pine wood nematode is a devastating forest disease that mainly harms Pinus pla...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01P5/00
Inventor 吴小芹朱丽梅叶建仁徐旭凌陈江天
Owner NANJING FORESTRY UNIV
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