Novel device for removing inner filtering effect in fluorimetric determination and novel testing method

Abstract

The invention discloses a novel device for removing the inner filtering effect in fluorimetric determination and a novel testing method. The method determines the actual influencing degree of the inner filtering effect through experiments so as to remove the influence of the inner filtering effect. The method has simple and convenient operation, economy, applicability and reliable results and can solve the problems existing in the prior method. A structure of the device comprises a light path device which consists of a sample pool and an absorption pool and is used for a fluorospectrophotometer; the absorption pool of the light path device has an L shape; the sample pool has a square shape; the L-shaped absorption pool semi-encircles the sample pool; and the width of the sample pool is twice of that of the absorption pool.

Description

technical field [0001] The invention belongs to the field of analysis science and technology, and relates to a new method based on a specially designed sample pool and corresponding testing to eliminate the inner filter effect in the fluorescence measurement process, and especially relates to a new optical path system for eliminating the inner filter effect in the fluorescence measurement. Device and new method of testing it. Background technique [0002] After certain substances absorb photons with the same frequency as its own characteristic, the electrons in its atoms are excited to a higher energy level, thereby producing an absorption spectrum. When some substances are irradiated with light, they absorb light of a certain wavelength and emit light of various colors and intensities; when the light is stopped, this light also disappears. This light is called Fluorescence. The wavelength of fluorescence emitted is longer than the wavelength of the specific light absorbed...

AI Technical Summary

Problems solved by technology

This phenomenon is negligible at low concentrations of non-fluorescent substances, but can have serious effects at high concentrations

In order to eliminate this effect, the empirical formula is usually used to eliminate it, but the results often deviate from the real situation and the applicable concentration range is limited

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