Method for preparing tobacco extract and application thereof
A tobacco extract and extract technology, applied in the field of phytochemistry, can solve the problems of increased roughness and dryness, decreased quality of cigarettes, and increased smoke aroma, etc., and achieve high raw material utilization, low extraction temperature, and reduced damage Effect
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Embodiment 1
[0014] (1) Enzymolysis: In an ultrasonic extraction tank with stirring and jacketed water bath heating device, add 1000g of tobacco powder, 5000g of deionized water, add citric acid, adjust the pH value of the system to 4.5, add cellulase, and Enzymolysis at ℃ for 12 hours, stirring for 10 minutes every 30 minutes during enzymolysis;
[0015] (2) Ultrasonic extraction: After the enzymolysis is completed, add 7000g of deionized water again, and extract with ultrasonic wave at 50°C for 60 minutes, collect the first extraction, and use the same method to extract the residue again, collect the second Extraction solution;
[0016] (3) Centrifugal filtration: the above two extracts were combined, and centrifuged at room temperature at 1600 rpm for 20 minutes to obtain a clarified extract;
[0017] (4) Chromatographic column separation: the clarified extract after centrifugation is adsorbed on a clean chromatographic column, and 10L of extract is adsorbed per minute. During the adso...
Embodiment 2
[0021] (1) Enzymolysis: In an ultrasonic extraction tank with stirring and jacketed water bath heating device, add 1000g of tobacco powder, 7000g of deionized water, add citric acid, adjust the pH value of the system to 5.5, add cellulase, Enzymolysis at 48°C for 9 hours, stirring for 10 minutes every 30 minutes during enzymolysis;
[0022] (2) Ultrasonic extraction: After the enzymolysis is completed, add 5000g of deionized water again, and use ultrasonic extraction for 40 minutes under the condition of 58°C to collect the first extraction, and use the same method to extract the residue again, and collect the second Extraction solution;
[0023] (3) Centrifugal filtration: the above two extracts were combined, and centrifuged at room temperature at 1700 rpm for 20 minutes to obtain a clarified extract;
[0024] (4) Chromatographic column separation: the clarified extract after centrifugation is adsorbed on a clean chromatographic column, and 10L of extract is adsorbed per mi...
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