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Populus euphratica DREB transcription factor, encoding gene and application thereof

A Populus euphratica, amino acid technology, applied in application, genetic engineering, plant genetic improvement and other directions, can solve problems such as plant stress resistance

Active Publication Date: 2009-11-04
深圳市园林科学研究所 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a large number of genes related to stress resistance have been cloned from various plants, most of these genes can only increase a single resistance of plants, and cannot comprehensively improve the stress resistance of plants as a whole.

Method used

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  • Populus euphratica DREB transcription factor, encoding gene and application thereof
  • Populus euphratica DREB transcription factor, encoding gene and application thereof
  • Populus euphratica DREB transcription factor, encoding gene and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0057] Example 1 Isolation and Cloning of Populus euphratica DREB Transcription Factor (PeDREB2b) Gene

[0058] 1 Materials and methods

[0059] 1.1 Materials

[0060] 1.1.1 Plant material cultivation and stress treatment

[0061] Populus euphratica seedlings were collected in Lop Nur, Xinjiang, and are now planted in Langfang Experimental Base, Hebei Province. During sampling, short branches of Populus euphratica were cut and inserted into 20% PEG6000 solution to simulate drought stress for 10 hours, and the leaves were collected and treated with liquid nitrogen Store in an ultra-low temperature refrigerator for later use.

[0062] Treatment of different stress materials: Cut short branches of Populus euphratica, insert them into 250mM sodium chloride, 20% PEG6000, 100μM ABA solution, 100μM GA3 solution, 100μM BA solution, 100μM NAA solution and place them at 4°C They were processed in the refrigerator for 0.5, 3, 6, 12 and 24 hours. The processed materials were quickly f...

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Abstract

The invention discloses a DREB transcription factor cDNA sequence (PeDREB2b gene) which is separated and cloned from the psammophytes Populus euphratica and is closely relevant to stress resistance. The invention further discloses an efficient plant expression carrier pRCAMBIA2301-PeDREB2b containing the cDNA sequence and an application thereof in improving the stress resistance of plants. The invention uses Agrobacterium as a medium to transform the efficient plant expression carrier pRCAMBIA2301-PeDREB2b to tobacco; positive sprouts are screened and identified through Kan resistance, genome PCR and RT-PCR, and stress resistance such as drying and high salt and are analyzed, and relevant physiological and biochemical indexes are tested for the transgenic tobacco sprouts. The test result shows that the content of soluble sugar, cytoplasmic total protein and proline of tran-PeDREB2b gene plant is obviously greater than that of a contrast, so that the over-expression of PeDREB2b gene can obviously improve drought resistance and osmotic stress resistance of the transgenic tobacco.

Description

technical field [0001] The invention relates to the cDNA sequence of the plant AP2 / EREBP family transcription factor, in particular to the psammophyte Populus euphratica (Populuseuphratica) DREB2 transcription factor cDNA sequence. The invention also relates to a plant expression vector capable of efficiently expressing the cDNA sequence in plants and the application of the cDNA sequence in improving plant stress resistance, belonging to the field of plant genetic engineering. Background technique [0002] Drought, salinity and low temperature are three major abiotic stress factors that affect the growth of terrestrial plants and limit crop yield. According to statistics, the world's arid and semi-arid areas involve more than 50 countries and regions, accounting for about 34.9% of the global land area. At present, among the 5.7 billion hectares of arable dryland used for agriculture in the world, about 70% of the arable land is degraded due to factors such as drought. The l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82C12N5/10A01H1/00
Inventor 雷江丽吴燕民刘水谢良生未丽
Owner 深圳市园林科学研究所
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