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Clenobuterol hydrochloride, salbutamol and paylean three joint inspection card and method for processing detecting sample

A ractopamine triple detection card and clenbuterol hydrochloride technology, which is applied in material inspection products, measuring devices, biological tests, etc., can solve the problem of no simultaneous detection and detection equipment, etc., and achieves saving detection costs, convenient and fast use, and results. accurate effect

Inactive Publication Date: 2009-10-28
无锡安迪生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the detection of clenbuterol hydrochloride, albuterol and ractopamine in the prior art mainly relies on chemical instrument analysis and enzyme immunoassay kits. So far, there is no detection device that detects these three substances at the same time

Method used

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  • Clenobuterol hydrochloride, salbutamol and paylean three joint inspection card and method for processing detecting sample
  • Clenobuterol hydrochloride, salbutamol and paylean three joint inspection card and method for processing detecting sample
  • Clenobuterol hydrochloride, salbutamol and paylean three joint inspection card and method for processing detecting sample

Examples

Experimental program
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Effect test

Embodiment 1

[0016] Example 1: Clenbuterol hydrochloride, albuterol and ractopamine triple detection card

[0017] It is to set the test strip 2 in the test card shell 1, the structure of the test card shell 1 and the setting of the test strip 2 refer to the attached figure 1 , The detection card shell 1 is a long flat thin shell shell, 7cm long, 2cm wide, 0.5cm thick, and the thin shell wall thickness is 0.1cm, made of engineering plastics. The detection card casing 1 is composed of two halves of a casing cover and a casing seat, and a detection window 3 and a sample injection hole 4 are arranged on the casing cover of the detection card casing 1 . The test strip 2 is placed in the shell seat of the test card shell 1 .

[0018] The test strip 2 is a thin strip with a multi-layer structure, the length of the strip is about 6.0cm, the width of the strip is about 0.4cm, and the thickness is less than 2.5mm. The test strip 2 has a multilayer structure: the bottom layer is a supporting backb...

Embodiment 2

[0019] Embodiment 2 detects the processing method of sample

[0020] The clenbuterol hydrochloride, salbutamol and ractopamine triple detection card of the present invention is used to detect whether clenbuterol hydrochloride, salbutamol and ractopamine are contained in livestock feed or animal urine samples or animal tissues, meat and liver, and to identify livestock food accordingly security.

[0021] For different test samples, there are different processing methods:

[0022] The treatment method for urine samples is: the urine is directly used as a test sample. If the urine sample is turbid, it needs to be filtered through filter paper first, and the supernatant is taken as a test sample.

[0023] The treatment method for the feed sample is: weigh 0.5 g of the feed sample, add 1 mL of the sample extract (pH 7.050 mmol / L Tris-HCL), stir in the test tube for 1 minute, and after standing for 10 minutes, take the clear supernatant as a test sample.

[0024] The treatment me...

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Abstract

The invention relates to a clenobuterol hydrochloride, salbutamol and paylean three joint inspection card and a method for processing a detecting sample, and belongs to the technical field of detection of a beta-receptor stimulating agent, wherein a test strip is arranged inside a shell of the three joint inspection card, and the clenobuterol hydrochloride, salbutamol and paylean three joint inspection card is formed by the adhesion of a sample gasket, a colloidal gold membrane, a cellulose nitrate membrane and a water absorbing membrane to a bearing backboard in turn; the colloidal gold membrane is a glass fiber membrane of a colloidal gold marker containing a clenobuterol hydrochloride antibody, a salbutamol antibody and a paylean antibody; three detection strips are arranged on the cellulose nitrate membrane and contain clenobuterol hydrochloride protein conjugate, salbutamol protein conjugate and paylean protein conjugate respectively; and a quality control strip containing an anti-rabbit antibody or an anti-mouse antibody is arranged. The clenobuterol hydrochloride, salbutamol and paylean three joint inspection card has the advantages of simultaneously detecting the clenobuterol hydrochloride, the salbutamol and the paylean in urine or feed, animal tissue, meat and liver. The inspection card is easy to prepare and quick and convenient to use, saves the detection cost, and has accurate result.

Description

technical field [0001] The invention relates to a device for detecting β-receptor agonists, in particular to a clenbuterol hydrochloride, salbutamol and ractopamine triple detection card and a processing method for detection samples thereof. The invention belongs to the technical field of beta-receptor agonist detection. Background technique [0002] β-receptor agonists are a kind of synthetic adrenal receptor agonists, including clenbuterol hydrochloride (clenbuterol), salbutamol (Salbutamol) and ractopamine (ractopamine), commonly known as "leptin", which can promote animal Decompose poultry fat, increase muscle growth, and act as a nutrient redistribution agent to increase lean meat percentage, reduce fat deposition and promote animal growth. It is illegally used by some livestock farmers as a breeding accelerator. Incidents of food poisoning of consumers caused by the illegal use of β-agonists in livestock feed have occurred from time to time. Clenbuterol, as a feed ad...

Claims

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Application Information

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IPC IPC(8): G01N33/552G01N33/531G01N33/558G01N33/52
Inventor 周坚
Owner 无锡安迪生物工程有限公司
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