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Gene expression down-regulated vector of cell specificity ndrg2

A technology of gene expression and transgene carrier, which is applied in the field of cell-specific ndrg2 gene expression down-regulation carrier, which can solve the problems of non-tissue cell specificity and achieve obvious effect of down-regulation

Inactive Publication Date: 2009-10-28
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

[0013] Currently, RNAi transgene vectors used in RNAi transgenic animals to down-regulate the expression of target genes use a polymerase III-dependent promoter (CMV) upstream of the miRNA junction site (miR flanking region). The interfering sequence that initiates the access of the downstream miRNA junction site in the cell, so the down-regulation of the target gene expression is not tissue-cell specific

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  • Gene expression down-regulated vector of cell specificity ndrg2
  • Gene expression down-regulated vector of cell specificity ndrg2
  • Gene expression down-regulated vector of cell specificity ndrg2

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[0042] MicroRNA (miRNA) is a kind of eukaryotic endogenous small molecule single-stranded RNA, usually 18-25 bp in length, which can cause the degradation of target mRNA or inhibit its translation through specific base pairing with target mRNA, thereby affecting Post-transcriptional expression regulation of genes is an important molecular tool in RNA interference. In recent years, hundreds of miRNAs have been discovered in animal cells and plant tissues. These small molecular regulatory RNAs are matured by cutting out the 60-200bp precursor with a hairpin structure.

[0043] In animal cells, the primary product of miRNA gene transcription (pri-miRMA) is quickly processed into miRNA precursor (pre-miRNA) by ribonuclease III Drosha (RNaseIII Drosha), and then transported from the nucleus to the cytoplasm, through another An RNaseIII Dicer recognizes spliced ​​mature miRNAs. These mature miRNAs and other proteins together form the RISC complex (RNA-induced silencing complex), wh...

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Abstract

The invention discloses a gene expression down-regulated vector of cell specificity ndrg2, which comprises an RNAi transgenic vector. A preproinsulin promoter is connected to the RNAi transgenic vector, a miR accessed locus is inserted to the down stream of the preproinsulin promoter, and the miR accessed locus is connected with a miRNA sequence aiming at down-regulated ndrg2 genes. The constructed RNAi transgenic vector adopts the preproinsulin promoter relied by RNA polymerase II to promote the downstream miRNA sequence by specific insulin beta cells of the preproinsulin promoter, and the specific insulin beta cells promote RNA interference aiming at ndrg2 genes to down-regulate the expression thereof; and the vector is used for constructing an animal model and researching the functions of the ndrg2 genes of the insulin beta cells.

Description

technical field [0001] The invention belongs to the technical field of gene function related to type II diabetes, relates to the gene function research of type II diabetes related gene ndrg2, and particularly relates to a carrier for down-regulating the expression of cell-specific ndrg2 gene. Background technique [0002] ndrg2 and its specific expression in tissue cells [0003] The human ndrg2 gene (N-Myc Downsteam-Regulated Gene) is a new gene with low expression in tumor cells discovered and cloned by Deng Yanchun et al. (Deng Yanchun, Yao Libo, Liu Xinping. A new gene containing an ACP-like domain Discovery Biochemistry and Biophysics Progress. 2001, 28 (1): 72-76), because this gene has a relatively similar structure with the N-myc down-stream regulated gene ndrg1 (N-myc down-stream regulated gene 1) that has been discovered High homology, so it was named ndrg2. The full-length cDNA of the gene is 2024bp, encoding 357 amino acids, located at 14q12.1, with 16 exons an...

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Application Information

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IPC IPC(8): C12N15/85
Inventor 吴有盛刘新平药立波
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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