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Populus euphratica dreb2 transcription factor cDNA sequence, expression vector containing the sequence and application thereof

A plant expression vector, the technology of Populus euphratica, applied in the field of plant genetic engineering, can solve the problems of incapable plant stress resistance and the like

Inactive Publication Date: 2011-12-07
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a large number of genes related to stress resistance have been cloned from various plants, most of these genes can only increase a single resistance of plants, and cannot comprehensively improve the stress resistance of plants as a whole.

Method used

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  • Populus euphratica dreb2 transcription factor cDNA sequence, expression vector containing the sequence and application thereof
  • Populus euphratica dreb2 transcription factor cDNA sequence, expression vector containing the sequence and application thereof
  • Populus euphratica dreb2 transcription factor cDNA sequence, expression vector containing the sequence and application thereof

Examples

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Embodiment 1

[0060] Example 1 Isolation and Cloning of Populus euphratica DREB Transcription Factor (PeDREB2a) Gene

[0061] 1 Materials and methods

[0062] 1.1 Materials

[0063] 1.1.1 Plant material cultivation and stress treatment

[0064] Populus euphratica seedlings were collected in Lop Nur, Xinjiang, and are now planted in Langfang Experimental Base, Hebei Province. During sampling, short branches of Populus euphratica were cut and inserted into 20% PEG6000 solution to simulate drought stress for 10 hours, and the leaves were collected and treated with liquid nitrogen Store in an ultra-low temperature refrigerator for later use.

[0065] Treatment of different stress materials: Cut short branches of Populus euphratica, insert them into 250mM sodium chloride, 20% PEG6000, 100μM ABA solution, 100μM GA3 solution, 100μM BA solution, 100μM NAA solution and place them at 4°C They were processed in the refrigerator for 0.5, 3, 6, 12 and 24 hours. The processed materials were quickly f...

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Abstract

The invention discloses a DREB transcription factor cDNA sequence (PeDREB2a gene) which is separated and cloned from Populus euphratica and is closely related to stress resistance, wherein the Populus euphratica is a sand plant. The invention also discloses a high-efficiency plant expression carrier containing the cDNA sequence and an application thereof in improving the reverse resistance of plants. In the invention, the PeDREB2a gene is established into a plant high-efficiency expression carrier p2MDK-PeDREB2a; agrobacterium is utilized to carry out mediate transformation for tobaccos; positive seedlings are selected and identified by Kan resistance and genomes PCR and RT-PCR; the reverse resistance analysis of transgenic tobacco seedlings, such as drought, high salt, low temperature, and the like are carried out; and the correlative physiological-biochemical indexes of the transgenic tobacco seedlings are measured. A test result shows that the content of the dissoluble sugar, the cytoplast total protein and the praline of a PeDREB2a transgene plant strain is obviously higher than a contrast, so that the overexpression of the PeDREB2a gene can obviously improve the drought resistance and the infiltration stress resistance of transgenic tobaccos.

Description

technical field [0001] The invention relates to the cDNA sequence of the plant AP2 / EREBP family transcription factor, in particular to the psammophyte Populus euphratica (Populuseuphratica) DREB2 transcription factor cDNA sequence. The invention also relates to a plant expression vector capable of highly expressing the cDNA sequence in plants, and the effect of the cDNA sequence in improving plant stress resistance, belonging to the field of plant genetic engineering. Background technique [0002] Drought, salinity and low temperature are three major abiotic stress factors that affect the growth of terrestrial plants and limit crop yield. According to statistics, the world's arid and semi-arid areas involve more than 50 countries and regions, accounting for about 34.9% of the global land area. At present, among the 5.7 billion hectares of arable dryland used for agriculture in the world, about 70% of the arable land is degraded due to factors such as drought. The land area ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/11C12N15/29C12N15/82C12N5/10A01H1/00
Inventor 吴燕民雷江丽未丽梁哲唐益雄
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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