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Method for extracting highland barley Beta-dextran and dietary fiber by combined-enzyme method

An extraction method and glucan technology are applied in the fields of intensive processing and comprehensive utilization of highland barley by-products, which can solve the problems of β-glucan not being developed and utilized, and achieve the improvement of economic added value, broad application prospects and economic income. Effect

Inactive Publication Date: 2011-05-11
HENAN UNIVERSITY OF TECHNOLOGY
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AI Technical Summary

Problems solved by technology

Nutrients in highland barley, especially β-glucan and dietary fiber in highland barley bran, have not been effectively developed and utilized

Method used

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Effect test

Embodiment Construction

[0025] The highland barley bran is first processed by a bran beating machine, then sieved to remove the sticky flour in the bran, and then crushed through a 40-mesh sieve. Enzymatic hydrolysis: After pulverizing the bran, the ratio of material to water is 1:6, adjust the pH to 5 with dilute hydrochloric acid, add cell wall decomposing enzyme (xylanase, cellulase or pentosanase, 40u / 100mL), at 45°C Enzymolyze for 1 hour, centrifuge, and then use an extruder to process the bran. On the one hand, the enzyme is eliminated, and on the other hand, the structure of the bran is further loosened, which is convenient for subsequent extraction and separation. Then, it is crushed through a 40-mesh sieve, and 30g of crushed bran is weighed. Put the leather sample into a reactor equipped with a stirring device, add 300mL of water, 120u of cellulase, stir in a 45°C water bath for 1h, then adjust the pH to 10 with anhydrous sodium carbonate, and stir in a 45°C water bath for 1h; take out the r...

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Abstract

The invention discloses a method for extracting a Beta-dextran in highland barley bran and preparing a highland barley dietary fiber, belonging to the field of food processing. The method comprises the following steps of: extracting and centrifuging the highland barley bran taken as raw material at an alkaline condition by zymohydrolysis of secondary cellulose, thus obtaining a deposition A and asupernatant; depositing and centrifuging the supernatant under an acidic condition to remove albumen; adding a high-temperature resistant Alpha-amylase into the supernatant under a neutral condition for zymohydrolysis; centrifuging, depositing in water, depositing and re-dissolving the supernatant by water, centrifuging and taking the supernatant; repeatedly depositing the supernatant by a ammonium sulphate and obtaining the Beta-dextran product; adding a composite enzyme in the deposition A for zymohydrolysis; adding a hydrogen peroxide in the deposition A for decoloring; washing the deposition A repeatedly by water; obtaining a solid by centrifuging; and drying the solid at low temperature to obtain the highland barley dietary fiber. The method can sufficiently utilizes the highland barley bran, obtains two products of Beta-dextran and dietary fiber, effectively improves the economical additional value of the highland barley bran and has important realistic significance and wide application prospect for developing high-tech industries.

Description

Technical field: [0001] The invention relates to the extraction of β-glucan in highland barley and the preparation method of highland barley dietary fiber, relates to the intensive processing and comprehensive utilization technology of highland barley by-products, and belongs to the field of food processing. Background technique: [0002] Highland barley is an important food crop that grows in the northwest and southwest of my country, especially Tibet, Qinghai and other places. Highland barley has the characteristics of high protein, high fiber, high vitamin, low fat and low sugar, and is a good product among cereal crops. Moreover, the content of β-glucan in highland barley is between 3.66% and 8.62%, with an average value of 5.25%, which is the highest value measured so far in cereal crops, much higher than that of barley, wheat, and oats. In recent years, a large number of studies have shown that highland barley β-glucan not only has the effects of lowering blood fat, lo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/02A23L1/09A23L1/30A23L1/308A23L1/10A23L1/28C12S3/02A23L33/125A23L33/21
Inventor 郑学玲李利民曲良冉
Owner HENAN UNIVERSITY OF TECHNOLOGY
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