Method for extracting ursolic acid from vegetable Dian clove

Abstract

The invention relates to a method for extracting ursolic acid from vegetable Dian clove. The method comprises the following steps that: (1) the dried Dian clove is picked up, crushed and undergoes repeated cold soaking by 10L of 95 percent ethanol for three times, seven days for each time, and the ethanol is filtered, decompressed and reclaimed to obtain a concentrated extractive; (2) the obtained ethanol extractive is tested; and (3) the extract obtained from the step(2) is subjected to Sephadex LH-20 gel column chromatography and is separated to obtain the finished product by taking methanol as an eluant and compare with the standard product. The separation materials used by the method include macroporous adsorptive resin and gel, so that the separation materials have stable physical-chemical properties, are not dissolved in acids, alkalis and organic dissolvants, have good selectivity on organic matters, and are not affected by existence of organic salts and strong-ion low-molecule compounds; and the separation materials are easily eluted and regenerated, thereby facilitating the cyclic utilization.

Description

technical field [0001] The invention relates to a method for extracting ursolic acid from plants, in particular to a method for extracting and separating ursolic acid from Rubiaceae plant Lilac yunnanensis. Background technique [0002] Ursolic acid (ursolic acid, UA), also known as ursolic acid, is widely distributed in nature. It exists in the leaves and fruits of the rhododendron bearberry plant, the leaves of the plant Paulownia tomentosa, and the fruit of the Rubiaceae plant Gardenia , Gentianaceae wet buds, Oleaceae privet leaves and other plants. Ursolic acid has a wide range of biological effects, and was listed as one of the most promising tumor prevention drugs by Japan in 1990. It has obvious stabilizing and cooling effects on the central nervous system; it has antibacterial activity against G+ and G- bacteria and yeast in vitro; it has anti-diabetic, anti-ulcer and blood-lipid-lowering effects; it is resistant to various carcinogens and carcinogens Function; it...

AI Technical Summary

Problems solved by technology

Under normal circumstances, ursolic acid is synthesized by chemical methods, and there are "three wastes" in the chemical synthesis, which has a large amount of treatment and is difficult to deal with, and it is easy to pollute the environment and the cost is high

So far, there are many reports about extracting and isolating ursolic acid from other plants, but most of them are extracted from Oleaceae plants and rhododendron plants, and there is no report on obtaining ursolic acid from the Rubiaceae plant Dian lilac.