Preparation method of tissue engineering xenoskin

Abstract

The invention relates to a preparation method of tissue engineering xenoskin, which adopts a baby pig with rich source as a skin source to obtain the finished product through the steps of taking the skin, fixing the skin, cutting, removing hypodermal cells, modifying three-dimensional collagenic structure, changing physical characteristics and sterilizing and packaging, and the finished product can be stored for more than 2 years under the environment of 2-10 DEG C. The tissue engineering xenoskin is applied to deep burn and defect of skin, has the functional characteristics of low immunogenicity, compact adhesion on wound surface, and moisture maintenance, can be used as a protection wound surface of a provisional wound surface cover of a human body, and can also be used as a growth repair wound surface of permanently remaining corium bracket. The invention solves the defects of high cost, limit source, long fabrication time, short storage time and organism hurt by secondary skin-grafting in the prior artificial skin product. The prepared product material has wide material source, short product period, short storage time and low cost, and is widely applied to the clinic.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering biological materials, and in particular relates to a preparation method of tissue engineering heterogeneous skin. Background technique [0002] Severe deep burns and skin defects need to solve the problem of fast and effective wound closure. At present, autologous skin grafting is mainly used in the clinical treatment of deep burns, which will increase new wounds. For patients with extensive burns, there is a problem of insufficient autologous skin sources. The transplantation technique of allogeneic skin plus autologous micro-skin is the most commonly used effective method to cover large-area deep burn wounds. However, the source of allogeneic skin is becoming more and more limited. This skin substitute is crucial in the treatment of patients with extensive deep burns. Large-area skin defects caused by severe trauma also need to face the above problems. In order to overcome these di...

AI Technical Summary

Problems solved by technology

Animal experiments and clinical practice have proved that pigskin dressings have the following defects: porcine acellular dermal matrix lacks epidermal structure, and autologous blade-thick skin is still required to cover it when applied, and a second autologous skin transplantation is required to form new damage to the patient; Fresh pigskin has strong antigenicity and a high rate of bacterial contamination. It is used to cut (cut) the scab and reject it early, and it is easy to induce infection. The exposed wound requires a second operation; glutaraldehyde pigskin has poor elasticity and is water-permeable. High resistance, easy to dry after being applied to the wound, unable to adhere tightly to the wound, and slow growth of new blood vessels in the skin; the same as the freeze-dried pig skin, the irradiated pig skin has poor adhesion and has no effect on promoting the growth of the epidermal cells and dermal cells of the body

Although these dressings can play a role in protecting the wound surface, they all have disadvantages in varying degrees. They cannot replace the allograft skin for covering the excision (cutting) scab wound surface of a large area of ​​deep burn, and can only be used as a temporary covering.