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Soap pod saponin extract as well as preparation method and application thereof

A saponin and extract technology, applied in the field of saponin extract and preparation thereof, can solve problems such as total saponins not yet found, and achieve the effects of promoting phagocytosis and prolonging survival time

Inactive Publication Date: 2009-09-23
SHANDONG ACAD OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pig tooth soap and large saponins are rich in saponins, and nineteen pentacyclic triterpene saponins have been isolated and identified from the n-butanol part of pig tooth soap (J.Nat.Prod.1999, 62 (5) : 740-745; Chem.Pham.Bull.1999,47(3):388-393; J.Nat.Prod.1999,62(6):877-881; Phytochemistry, 1999,52:715-722), So far, no relevant reports have been found on the clinical use of total saponins of saponins in the treatment of cancer and leukemia.

Method used

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  • Soap pod saponin extract as well as preparation method and application thereof
  • Soap pod saponin extract as well as preparation method and application thereof
  • Soap pod saponin extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the preparation of saponin extract of saponins

[0024] (1) Extraction of total saponins of acacia saponins: take the dried medicinal materials of the pig tooth soap, crush them into small pieces, weigh 5.0kg, add 75% ethanol to extract 3 times, each time the amount of alcohol added is 8mL / g medicinal materials, and the extraction time is 2 hours, filtered, combined the three extracts, recovered the solvent and concentrated under reduced pressure to a relative density of 1.20-1.35 (measured at 50°C) to obtain a thick paste, which is the crude saponin extract. Three parallel experiments were done, and the yield, yield and total saponin content of the crude extract are shown in Table 1.

[0025] Table 1 The amount and yield of total saponins extracted from pig tooth soap in three parallel experiments

[0026]

[0027] (2) Separation and purification of saponin crude extract

[0028] ①Preparation of samples for loading on the column: Weigh 120g of...

Embodiment 2

[0035] Embodiment 2: the preparation of saponin extract of saponins

[0036] (1) Extraction of total saponins of saponins: take the dried medicinal materials of saponins, crush them into small pieces, weigh 5.0kg, add 75% ethanol to extract 3 times, the amount of alcohol added each time is 8mL / g medicinal materials, and the extraction time is After 2 hours, filter, combine the three extracts, recover the solvent and concentrate under reduced pressure to a relative density of 1.20-1.35 (measured at 50°C), to obtain a thick paste, which is the crude saponin extract. Three parallel experiments were performed, and the yield, yield and total saponin content of the crude extract are shown in Table 3.

[0037] The amount and yield of three batches of total saponins extracted from Table 3

[0038]

batch number

Feed (Kg)

Yield of crude extract

(Kg)

Yield (%)

Total saponin content (%)

1

5.0

2.09 ...

Embodiment 3

[0042] Embodiment 3: the preparation of saponin extract of saponins

[0043] (1) Extraction of total saponins of saponins: get 5.0 kg of pig tooth soap, dry, pulverize into small pieces, extract 4 times with 25% ethanol, each time adding alcohol is 6mL / g medicinal material, extraction time is 3 hours, filter , combined alcohol extracts, concentrated under reduced pressure to a relative density of 1.20 to 1.35 (measured at 50°C), to obtain a thick paste, which is the crude extract of saponins;

[0044] (2) Separation and purification of saponin crude extract: get the above-mentioned saponin crude extract 0.5kg, add water 1.5L to dissolve, filter, collect the filtrate, and load the sample on AB-8 macroporous adsorption resin column (sample saponin crude extract and The weight ratio of the resin is 1:10), first eluted with deionized water, eluted to nearly colorless, then successively eluted with 20% ethanol, 40% ethanol, 60% ethanol, 80% ethanol, combined 40%, 60% % and 80% eth...

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Abstract

The invention discloses a soap pod saponin extract, a preparation method thereof and an application for curing lung cancer, liver cancer, stomach cancer, colorectal cancer and leukemia. The soap pod saponin extract uses fructus gleditsiae or fructus gleditsiae sinensis as the raw material and is prepared by the extraction of ethanol, and the separation and the purification of macroporous resin, and the content of total saponin of the extract is more than 60 percent. The saponin extract mainly comprises various pentacyclic triterpene saponin compounds, has obvious lethal effect on the clone of the leukemia progranulocyte HL-60 and the stomach cancer transferring lymphocyte SGC7901 of people, and has remarkably inhibited effect on the tumor of mice S180 and mice having the liver cancer. When capsules prepared by the extract are used for curing 40 examples having liver cancer, lung cancer, stomach cancer and colorectal cancer, the partial remission rate is 45 percent, and the partial remission and stabilization rate is 72.5 percent. When the capsules are used for curing 30 patients who have leukemia, the complete remission rate is 66.7 percent, and the total remission rate is 90 percent which is better than that of a single chemo-treatment group. The extract can obviously lighten the toxic side effect caused by the chemo-treatment.

Description

technical field [0001] The invention relates to a saponin extract of saponins and its preparation method, as well as its application in the preparation of medicines for treating liver cancer, lung cancer, stomach cancer, colorectal cancer or leukemia. Background technique [0002] Cancer and leukemia (also known as blood cancer) are malignant diseases that seriously threaten human life, accounting for the first and second place in the death toll. However, there are still no ideal therapeutic drugs and methods at present. At present, patients with early, middle and advanced cancers often use surgery, surgery plus radiotherapy, chemotherapy, surgery plus Chinese medicine and radiotherapy, chemotherapy plus Chinese medicine and other different therapeutic methods. These methods Although it has a certain therapeutic effect, can partially relieve symptoms and prolong the life of patients, radiotherapy and chemotherapy can easily cause serious trauma to the human body. Among the e...

Claims

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Application Information

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IPC IPC(8): A61K36/483A61P35/00A61K131/00
Inventor 贾元印赵渤年李贵海曹芳刘善新梁瑞雪张新军唐由君
Owner SHANDONG ACAD OF CHINESE MEDICINE
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