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Visual storing method for long-term storage of DNA molecules and a device thereof

A DNA molecule, long-term preservation technology, used in DNA preparation, recombinant DNA technology, biochemical cleaning devices, etc., to achieve artistic effects

Inactive Publication Date: 2009-09-16
樊世斌 +1
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Problems solved by technology

[0002] At present, long-term preservation of DNA molecules is mostly used in the fields of practical medicine and scientific research. On the premise of long-term preservation of the stability of genetic material, the stored DNA can be visualized when needed, and its storage space is designed as an art with individual characteristics. Modeling to make it artistic and ornamental, there is no relevant report yet

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  • Visual storing method for long-term storage of DNA molecules and a device thereof

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[0019] Materials: Human peripheral blood anticoagulated with EDTA, or gargle with 50ml of normal saline or scrape with a cotton swab to get oral mucosal cells, or scrape skin epidermal cells, or take a small amount of hair, or take body tissue or organ specimens through surgery.

[0020] DNA extraction: Extract DNA by conventional alkaline lysis method or genome-wide DNA extraction kit, separate and purify DNA by phenol / chloroform, and precipitate DNA by absolute ethanol (stable for long-term storage).

[0021] Preservation: After volatilizing ethanol, the dried DNA was redissolved in Tris hydrochloride-EDTA (TE) buffer, and a low-melting point agarose gel was prepared. EB (concentration: 10ug / ml) was added to the gel, and the DNA was separated by electrophoresis after loading. Observe the DNA band under the light, cut off the red DNA-containing agarose gel with a scalpel, heat (about 60-70°C) to melt the gel, and pour the DNA-containing gel solution into the cavity of the pres...

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Abstract

The invention relates to a visual storing method for long-term storage of DNA molecules and a device thereof. Currently, the long-term storage of the DNA molecules is mainly applied to the fields of practical medicine and scientific research so as to lead the DNA molecules to have artistic quality and appreciation and meet memory requirement and relevant reports have not been released currently. The invention is a visual storing method for long-term storage of DNA molecules and a device thereof; the method comprises the following steps: (1) DNA is extracted; and (2) sol-gel solution containing DNA is poured into a cavity of the storing device, vacuumization is carried out after the sol-gel solution is cooled and solidified and inert gas is charged and then the cavity is sealed. The invention leads the stored DNA to be visualized when in need under the premise of the stability of long-term stored genetic material, designs the storage space thereof into artistic modeling with personalized features, realizes the effect of using long-term stored genetic material for medicine and the like, simultaneously has artistic quality, appreciation and meets requirements of memory.

Description

technical field [0001] The invention relates to a long-term and visualized preservation method and device for DNA molecules. Background technique [0002] At present, long-term preservation of DNA molecules is mostly used in the fields of practical medicine and scientific research. On the premise of long-term preservation of the stability of genetic material, the stored DNA can be visualized when needed, and its storage space is designed as an art with individual characteristics. Modeling, making it artistic and ornamental, has not yet seen relevant reports. Contents of the invention [0003] The object of the present invention is to provide a long-term and visualized preservation method and device for DNA molecules, which can artistically express individual DNA molecules. [0004] In order to achieve the above object, the technical scheme adopted in the present invention is: [0005] A method for long-term preservation and visualization of DNA molecules, which is specia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10C12M1/00
Inventor 樊世斌白玉杰
Owner 樊世斌
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