Method for comprehensively extracting and purifying oxidation-resistant active ingredient in sugarcane toppers or slag
An antioxidant activity, comprehensive extraction technology, applied in application, food preparation, food science and other directions, can solve the problems of underutilization of resources and stay in the theoretical research stage, etc., and achieve high development and application value, scientific and efficient methods, and resources. The effect of increased utilization
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Embodiment 1
[0024] 100 g of chopped sugarcane tip raw material was extracted twice with 60% ethanol with a solid-to-liquid ratio of 1:15 (mass to volume ratio) for 1 hour each time. Next, the raw material extract is passed through a chromatographic column filled with D101 macroporous adsorption resin, sugar and other water-soluble impurities are washed away with pure water, and then gradient elution is performed using ethanol aqueous solution with a concentration of 10-100% by volume. Collect the eluate with a concentration of 60% by volume, concentrate, use sodium hydroxide solution to adjust the pH value of the concentrated eluate to 10, use 3 times the volume of ethyl acetate to extract twice, and partially wash the sodium hydroxide solution with hydrochloric acid Adjust the pH value to 6, then extract twice with 3 times the volume of ethyl acetate, wash with water until neutral, then evaporate and concentrate under reduced pressure, freeze-dry, and determine the total flavonoids by cal...
Embodiment 2
[0026] 100 g of the extracted bagasse raw material was ultrasonically extracted 4 times with 60% methanol at a solid-to-liquid ratio of 1:6 (mass to volume ratio), 1 hour each time. Next, the raw material extract is passed through a chromatographic column filled with D301 macroporous adsorption resin, sugar and other water-soluble impurities are washed off with pure water, and then gradient elution is performed using ethanol aqueous solution with a concentration of 10-100% by volume. Collect the eluate with a volume percentage concentration of 70%, concentrate, use potassium hydroxide solution to adjust the pH value of the concentrated eluate to 9, use 1 volume of n-butanol to extract twice, and partially wash the potassium hydroxide solution with acetic acid Adjust the pH value to 5, then extract 3 times with 2 times the volume of n-butanol, wash with water to neutrality, then evaporate and concentrate under reduced pressure, freeze-dry, and determine the total flavonoids by c...
Embodiment 3
[0028] 100 g of chopped sugarcane tip raw material was extracted 3 times by percolation with 80% ethanol at a solid-to-liquid ratio of 1:8 (mass to volume ratio), 1 hour each time. Then, the raw material extract is passed through a chromatographic column filled with AB-8 macroporous adsorption resin, sugar and other water-soluble impurities are washed off with pure water, and then gradient washing is carried out with an ethanol aqueous solution with a concentration of 10 to 100% by volume. take off. Collect the eluate with a volume percentage concentration of 80%, concentrate, use sodium carbonate solution to adjust the pH value of the concentrated eluate to 9, use 2 times the volume of n-butanol to extract 3 times, and adjust the sodium carbonate solution part with formic acid to The pH value is 4, and then extracted 4 times with 1 times the volume of ethyl acetate, washed with water until neutral, then concentrated by evaporation under reduced pressure, freeze-dried, and det...
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