Molecular tagged method of indicating and identifying size of qin-chuan cattle body by using GDF5 gene

Abstract

The invention discloses a molecular tagged method of indicating and identifying the size of a qin-chuan cattle body by using GDF5 gene, which comprises the following steps: designing a pair of primers according to the cattle GDF5 gene order; utilizing the primers to perform PCR amplification to the genom DNA of the cattle; utilizing the PCR product gained and digested by restriction enzyme Mva I; and then using 2.5 percent of sepharose gel to perform the electrophoretic separation to the digested PCR product, detecting T / C variable sites of a 586 site of GDF5 gene exon 1; and respectively naming the variable sites of the GDF5 gene exon 1 of the cattle as C base, T base and T / C hybrid base. According to the experiment result, the length of the cattle with TT gene is 5.64-6.87cm less than that of the cattle with CC gene; and the hip width of the cattle with TT gene is 3.46-4.00cm less than that of the cattle with CC gene. The method has simple operation, low cost, high accuracy and rapid detection.

Description

technical field [0001] The invention belongs to the field of animal molecular genetics, and in particular relates to a molecular marker method for predicting and identifying the body size of Qinchuan cattle by using the GDF5 gene. A new method is provided. Background technique [0002] Qinchuan cattle are produced in the Guanzhong Plain in the Weihe River Basin in Shaanxi Province, and are named after "Eight Hundred Li Qinchuan". With Weinan, Pucheng in the east, Fufeng, Qishan and other 15 counties and cities in the west as the main production areas, it is one of the five fine-bred yellow cattle in China. Qinchuan cattle are famous all over the world for their strong stress resistance, resistance to rough feeding, and good meat quality, and are known as "the treasure of the country". Body size is an important index to determine the quality of breeds and production performance, and the study of cattle body size has become a prominent problem. In the long-term breeding pro...

AI Technical Summary

Problems solved by technology

In 1994, Chang et al. found that GDF5 was significantly expressed in the original cartilage of long bones, but was rarely expressed in vertebrae and ribs; a small amount of expression would cause the growth of limb bones to be hindered, affecting the body's body size; thereby reducing the GDF5 gene The transcription of the spine affects the growth of the spine, limb bones and connecting joints, resulting in smaller stature

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