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Human papilloma virus preventative vaccine, construction method and application

An anti-human papilloma and virus technology, applied in the direction of anti-viral agents, viral antigen components, chemical instruments and methods, etc., can solve the problems of acute and incomplete cure.

Inactive Publication Date: 2009-09-02
江阴艾托金生物技术有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

In developing countries, the death rate of most women from cervical cancer is next to that of breast cancer, and surgical treatment cannot completely cure

Method used

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  • Human papilloma virus preventative vaccine, construction method and application
  • Human papilloma virus preventative vaccine, construction method and application
  • Human papilloma virus preventative vaccine, construction method and application

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Experimental program
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Effect test

Embodiment 1

[0026] 1) Construction of chimeric protein expression plasmids:

[0027]The L1 gene sequences related to HPV6, 11, 16, 18, 33, 52, 58 were optimized according to Hansenula codons. Compared with the original wild-type gene nucleic acid sequence of the HPV virus, the homology of the optimized nucleic acid sequence is not less than 75%. For example, the homology compared with the wild type is: 76% for HPV6, 76% for HPV11, 77% for HPV16, 75% for HPV18, and 76% for HPV58. The optimized L1 gene sequence was sent to GenScript (New Jersey, USA) for synthesis. The products were identified, and the HPV6L1 gene with a full length of 1503bp, the HPV11L1 gene with a full length of 1516bp, the HPV16L1 gene with a full length of 1518bp, the HPV18L1 gene with a full length of 1524bp, and the HPV58L1 gene with a full length of 1497bp were synthesized. The synthetic gene fragment was first cloned into pUC57 plasmid. It is then fused with the synthesized small fragments of CTL-epitope and VL2...

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Abstract

The invention provides a construction method of a human papilloma virus resistant HPV polyvalent vaccine and application thereof.

Description

Technical field: [0001] The invention relates to a human papillomavirus vaccine produced by a molecular bioengineering method, which is used for preventing cervical cancer, other malignant tumors caused by HPV infection, and condyloma acuminatum related to venereal diseases. Specifically, the present invention relates to the selection and comprehensive use of gene fragments of viral targets to achieve the strongest antiviral effect. The invention also relates to modifying and optimizing the viral gene sequence to achieve the highest expression of the protein molecule in the eukaryotic host cell, and successfully preparing the vaccine antigen with immune activity. The anti-HPV virus vaccine involved in the present invention includes HPV subtypes: HPV6, 11, 16, 18, 33, 52, 58, their coat protein antigens L1, L2 and epitope short peptides with specific immune activity function. Background of the invention: [0002] Cervical cancer is a major problem in women's health all over ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61K38/08A61K38/16A61K39/12C07K19/00C12N15/62C12N15/79A61P31/20A61P35/00A61P37/00
Inventor 常小迦孙世章
Owner 江阴艾托金生物技术有限公司
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