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Dual-purpose bdellovibrio fermentation production process

A production process, technology of Bdellovibrio, applied in the field of fermentation, can solve the problems of low content of Bdellovibrio and short storage period

Inactive Publication Date: 2009-08-19
邱军强
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention provides a double-effect Bdellovibrio fermentation production process to overcome the problems of low content and short shelf life of bacteriophage Bdellovibrio produced by the existing technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Cultivation of the host bacterium Escherichia coli

[0018] 1. E. coli Incline Seed Culture

[0019] Solid medium (ordinary nutrient broth medium): peptone 0.5, beef extract 3.0, sodium chloride 0.5, agar 1.8, pH 7.0-7.2.

[0020] Inclined seed culture conditions: 24-36 hours at 37±1°C.

[0021] 2. Shake Flask Seed Culture

[0022] Shake flask seed medium: sodium chloride 0.5, yeast powder 0.3, peptone 0.5, pH 6.5-6.8. The first-level seed loading capacity is 100ml / 250ml△; the second-level seed loading volume is 200ml / 500ml△.

[0023] First-level shake flask seed culture: take 1 slant, add 10ml sterile water to make a bacterial suspension, and put 0.5ml / bottle into the seed bottle. Or take 1 slant, use the inoculation loop to pick 1~2 loops and put them into the seed bottle.

[0024] Secondary shake flask seed culture: Take the first-level seeds and put them into the seed bottle at a rate of 5ml / bottle.

[0025] Shake flask seed culture conditions: Shake flask cul...

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Abstract

The invention relates to the technical field of fermentation, in particular to a fermentation production technology of double-action Bdellovibrio. The invention provides a fermentation production technology of double-action Bdellovibrio, including the following steps: preparation of host strain turbid liquor; preparation of culture medium; and fermentation of double-action Bdellovibrio. The content of Bdellovibrio produced in the technology can reach over 5*108 pfu / mL, while the content of finished products produced by general factories is always less than 1*106 pfu / mL; for preservation period, the content of the Bdellovibrio of general factories is decreased by over two orders of magnitude after 3 to 6 months of storage; the order of magnitude of the products produced by the technology cannot be reduced within 12 months of storage, and is just reduced by one order of magnitude within 24 months of storage.

Description

technical field [0001] The invention relates to the technical field of fermentation, in particular to a fermentation production process of Bdellovibrio bieffectus. Background technique [0002] In aquaculture and animal disease prevention and control at home and abroad, chemical agents such as antibiotics are mainly used at present. The side effects of these preparations, such as drug residues, drug resistance of pathogenic bacteria, inhibition of beneficial bacteria, etc., have more and more adverse effects on human health and animal husbandry production. [0003] In recent years, the research and development of microbial ecological preparations has attracted the attention of all parties, especially the research on Bdellovibrio bacteriophage has been favored by people. Since Bdellovibrio phage was discovered in 1963, because it is a parasitic bacterium, it can lyse animal pathogenic bacteria such as Escherichia coli, Salmonella, Aeromonas hydrophila, Vibrio, etc. At the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/63
Inventor 邱军强
Owner 邱军强
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