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Biological polysaccharide, and production and use thereof

A biological polysaccharide and production method technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the problems of poor classification level, small production scale, inability to be widely used, etc., and achieve the effect of good moisturizing performance

Inactive Publication Date: 2009-08-12
张鹏 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the product obtained by the biological extraction method has high purity, it cannot be widely used due to the limitation of small production scale due to the small source of raw materials and high production cost.
There are disadvantages such as complex process, low product purity and poor classification level in the method of fermentation extraction, and the application is limited.

Method used

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  • Biological polysaccharide, and production and use thereof
  • Biological polysaccharide, and production and use thereof
  • Biological polysaccharide, and production and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] slant seed culture

[0073] Streptococcus zooepidemicus was inoculated on the slant medium.

[0074] Solid medium formula: 25 parts of sucrose, 3 parts of beef peptone, 5 parts of yeast powder, 2 parts of beef powder, KH 2 PO 4 4 parts, NaH 2 PO 4 2 parts, NaHCO 3 1 part, anhydrous MgSO 4 0.5 parts, 15 parts of agar and 1000 parts of water;

[0075] The technological conditions for preparing slant strains are: temperature 35° C., cultivation time 10 hours.

[0076] shake flask seed culture

[0077] Inoculate 1 ring of bacteria per 50ml of seed liquid culture medium.

[0078] Seed liquid medium formula: 20 parts of sucrose, 4 parts of beef peptone, 5 parts of yeast powder, 2 parts of beef powder, KH 2 PO 4 2 parts, NaH 2 PO 4 4 parts, NaHCO 3 1 part, anhydrous MgSO 4 0.5 parts and 1000 parts of water;

[0079] The technological conditions of seed liquor fermentation are: temperature 37°C, rotation speed 160r / min, cultivation time 10 hours.

[0080] fermen...

Embodiment 2

[0086] slant seed culture

[0087] Streptococcus zooepidemicus was inoculated on the slant medium.

[0088] Solid medium formula: 30 parts of sucrose, 4 parts of beef peptone, 3 parts of yeast powder, 4 parts of beef powder, KH 2 PO 4 2 parts, NaH 2 PO 4 4 parts, NaHCO 3 4 parts, 0.8 parts of anhydrous MgSO4, 15 parts of agar and 1000 parts of water;

[0089] The technological conditions for preparing slant strains are: temperature 37° C., culture time 15 hours.

[0090] shake flask seed culture

[0091] Inoculate 1 ring of bacteria per 50ml of seed liquid culture medium.

[0092] Seed liquid medium formula: 25 parts of sucrose, 5 parts of beef peptone, 3 parts of yeast powder, 3 parts of beef powder, KH 2 PO 4 4 parts, NaH 2 PO 4 1 part, NaHCO 3 4 parts, anhydrous MgSO 4 0.8 parts and 1000 parts of water;

[0093] The technological conditions of seed liquor fermentation are: temperature 35°C, rotational speed 170r / min, cultivation time 14 hours.

[0094] fermente...

Embodiment 3

[0100] slant seed culture

[0101] Streptococcus zooepidemicus was inoculated on the slant medium.

[0102] Solid medium formula: 20 parts of sucrose, 5 parts of beef peptone, 4 parts of yeast powder, 3 parts of beef powder, KH 2 PO 4 1 part, NaH 2 PO 4 1 part, NaHCO 3 2 parts, anhydrous MgSO 4 1 part, 15 parts of agar and 1000 parts of water;

[0103] The technological conditions for preparing slant strains are: temperature 30° C., culture time 13 hours.

[0104] shake flask seed culture

[0105] Inoculate 1 ring of bacteria per 50ml of seed liquid culture medium.

[0106] Seed liquid medium formula: 30 parts of sucrose, 3 parts of beef peptone, 4 parts of yeast powder, 4 parts of beef powder, KH 2 PO 4 1 part, NaH 2 PO 4 2 parts, NaHCO 3 2 parts, anhydrous MgSO 4 1 part and 1000 parts of water;

[0107] The technological conditions of seed liquor fermentation are: temperature 30°C, rotational speed 150r / min, cultivation time 15 hours.

[0108] fermenter cultu...

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Abstract

The invention relates a novel biological polysaccharide, a method for producing the same and application of the same. The biological polysaccharide is prepared by fermenting Streptococcus zooepidemicus CGMCC No. 1988. The preparation method comprises the following process steps of preparation of an inclined plane seed, preparation of seed solution, optimization of fermentation condition, separation purification of products and the like. The biological polysaccharide produced by the method has the advantages of high yield, simple process step, low production cost and the like; and in a liter of fermentation liquid, the content of the polysaccharide is between 20 and 50g, and the whole yield reaches between 70 and 90 percent. The research on the structure and the physical property of the biological polysaccharide shows that the biological polysaccharide comprises a plurality of disaccharide folding structures adopting a connection mode shown in the figure 1, has the characteristics of the macro molecular net structure biological polysaccharide, can be widely applied in various fields such as medical treatment, foods, cosmetics, oil extraction, biological materials and the like, and has important realistic significance.

Description

technical field [0001] The invention relates to a fermentation method for synthesizing desired compounds, and specifically belongs to a biological polysaccharide, a production method thereof, and an application of the polysaccharide. technical background [0002] With the continuous development and progress of biotechnology, more and more people pay attention to biological polysaccharides. At present, the common biological polysaccharides mainly include fungal polysaccharides and bacterial polysaccharides. The study of fungal polysaccharides began in the 1950s. After the 1960s, it aroused the interest of scientists because of its immune-promoting effect. People gradually discovered that it can not only resist bacteria, resist viruses, prevent and treat tumors, etc. Radiation, anticoagulant, hypoglycemic and other functions to regulate human physiological functions. Many fungal active polysaccharides, such as Lentinan, Poria cocos polysaccharide, Yunzhi polysaccharide, Schiz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12N1/20A61K31/715A61K8/73A23L1/09C09K8/04C02F1/00C12R1/46C02F101/32A23L29/30
Inventor 张鹏张淑荣徐永斌刘春巧陈畅
Owner 张鹏
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