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Silk fibroin porous three-dimensional material and preparation method thereof

A silk fibroin, three-dimensional technology, applied in medical science, prosthesis, etc., can solve the problem of small pore size

Active Publication Date: 2009-08-12
苏州盛泽科技创业园发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The porous material made of superimposed fibers obtained by electrospinning has a small pore size, which may be limited when used as a biomedical material

Method used

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  • Silk fibroin porous three-dimensional material and preparation method thereof
  • Silk fibroin porous three-dimensional material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation steps of the silk fibroin porous three-dimensional material provided in this embodiment are as follows:

[0023] 1. Put 1 kg of cocoon shells into 50 liters of neutral soap solution with a mass concentration of 0.5%, and treat them at 98-100°C for 2 hours to degumming the cocoon shells, and obtain pure silk fibroin (silk fibroin) after fully washing . The dried pure silk fibroin is dissolved in 5 liters of 9.3 mol / liter lithium bromide aqueous solution with stirring at 65±2° C. to form a silk fibroin mixed solution.

[0024] 2. Using a cellulose membrane as a dialysis material, dialyzing the obtained silk fibroin mixed solution with deionized water to remove impurities such as lithium bromide to obtain a pure silk fibroin solution.

[0025] 3. Adjust the mass concentration of the silk fibroin solution to 5%.

[0026] 4. Pour the silk fibroin solution into a stainless steel box, and quickly freeze at -30°C for 5 hours to obtain a silk fibroin solution f...

Embodiment 2

[0033] Put 1 kg of raw silk into 30 liters of sodium carbonate aqueous solution with a mass concentration of 0.2%, treat it at 98-100°C for 2 hours, degumming the raw silk, and obtain pure silk fibroin (ie silk fibroin) after fully washing. The dried pure silk fibroin is heated and dissolved at 75±5°C with 8 liters of calcium chloride, water and ethanol solution with a molar ratio of 1:8:2 to obtain a silk fibroin mixed solution.

[0034] The cellulose membrane is used as a dialysis material, and the obtained silk fibroin mixed solution is dialyzed with deionized water to remove impurities such as calcium chloride to obtain a pure silk fibroin solution.

[0035] Adjust the mass concentration of silk fibroin solution to 2%.

[0036] The silk fibroin solution was poured into an aluminum box, and quickly frozen at -60°C for 20 hours to obtain a silk fibroin solution frozen body.

[0037] Store the frozen body in a refrigerator at -10°C, and freeze and crystallize for 60 days.

[...

Embodiment 3

[0041] Put 100 grams of waste silk into 4 liters of sodium carbonate aqueous solution with a mass concentration of 0.05%, degumming at 98-100° C. for 0.5 hours, repeat the treatment 3 times, and obtain pure silk fibroin (ie silk fibroin) after sufficient washing. The dried pure silk fibroin is dissolved in 50 ml of 9.3 mol / L lithium bromide aqueous solution with stirring at 65±2° C. to form a mixed silk fibroin solution.

[0042] The cellulose membrane is used as a dialysis material, and the obtained silk fibroin mixed solution is dialyzed with deionized water for 3 days to obtain a pure silk fibroin solution.

[0043] Concentrate with 10% polyethylene glycol solution to obtain a silk fibroin solution with a mass concentration of 15%.

[0044] The silk fibroin solution was poured into a stainless steel box, and quickly frozen at -20°C for 10 hours to obtain a silk fibroin solution frozen body.

[0045] Store the frozen body in a refrigerator at -5°C, and freeze and crystalliz...

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Abstract

The invention relates to a porous material and a preparation method thereof, in particular to a technology in which silk fibroin is taken as a raw material to prepare porous three-dimensional materials. In the method, silk fibroin solution is injected into a metal mould, 1-24 hours of quick freezing is carried out on the solution at the low temperature of minus 10-minus 80 DEG C, thus obtaining afrozen body; the frozen body is preserved at the temperature of minus 5-minus 25 DEG C for 2-60 days, thus obtaining frozen crystalline solid; then the frozen crystalline solid is thawed and dried, thus obtaining the silk fibroin porous three-dimensional material. The silk fibroin of the porous material is fibroin I type crystal structure, therefore, the silk fibroin is insoluble in water and canbe completely degraded in a while and is finally degraded into nonpoisonous amino acid; the material of the invention has mutual run-through pores with aperture of 10-500 microns; the pores and pore diameter are relatively large, thus being suitable for growth of cells; meanwhile, in the process of preparation, no chemical cross linking agent, foaming agent or denaturing agent are needed, thus retaining fine biocompatibility of the silk fibroin.

Description

technical field [0001] The invention relates to a porous material and a preparation method thereof, in particular to a technique for preparing a porous three-dimensional material using silk fibroin as a raw material. The prepared material can be applied to technical fields such as biomedicine, biotechnology, and tissue engineering. Background technique [0002] Biomedical materials, especially tissue engineering scaffold materials, tissue repair regeneration materials, etc., require that the used biomaterials have good biocompatibility, and must also have certain bioactivity, biodegradability and three-dimensional porous structure. [0003] Silk is composed of about 75% silk fibroin and about 25% sericin, and is a fairly pure natural protein fiber. Studies have shown that silk fibroin is non-toxic, non-irritating, has good biocompatibility, and has certain biodegradability. In recent years, comparative studies on various biological materials have shown that silk fibroin has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/56A61L27/58
Inventor 卢神州李明忠
Owner 苏州盛泽科技创业园发展有限公司
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