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System and compositions for isolating suppressors of HIV-1 protease

A protease inhibitor, protease substrate technology, applied in the field of molecular biology and medicine, cell biology

Inactive Publication Date: 2009-08-05
SUZHOU SIRNAOMICS BIOPHARMACEUTICALS CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One of the main problems faced by ART therapy is that the virus can quickly develop resistance to the drug. The PI resistance of the virus is generally due to the gradual accumulation of mutations in the protease gene, and this resistance grows rapidly

Method used

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  • System and compositions for isolating suppressors of HIV-1 protease
  • System and compositions for isolating suppressors of HIV-1 protease
  • System and compositions for isolating suppressors of HIV-1 protease

Examples

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Embodiment approach

[0174] Fission yeast killing after expression of the HIV-1 protease gene from the nmt1 promoter of the plasmid is what we discuss here. This ability to kill fission yeast is based on screening for protease inhibitors and is a special field of this invention. Although the method of killing yeast by expressing a protease gene in a plasmid is the first to be demonstrated, because transfecting a plasmid is easier to handle than integrating a gene into a chromosome, and of course, in some respects, inhibiting by self-replication of the plasmid Physical screening is not the best option. Plasmids in fission yeast are extremely unstable compared to chromosomes, and integration of genes into chromosomes has the advantage of intrinsic stability. Gene integration can lead to more uniform expression, because the plasmid replication factor varies from 0-30 times, resulting in large changes in gene expression levels in the plasmid.

[0175] Unlike plasmid genes, the obvious advantage of u...

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Abstract

The present invention relates to a yeast screening assay for the identification of suppressors of a protease, such as the HIV-I protease and / or HIV-2 protease, for example. Upon overexpression of HIV-I protease in Schizosaccharomyces pombe, for example, the cell dies, but in the presence of a candidate inhibitor with protease suppressor activity, the cell lives, thereby providing an assay for the identification of one or more suppressors. In additional aspects, there is an in vivo protease activity screen, used alternatively or additionally to a viability screen, such as for cleavage of a HIV-I protease-specific site. In further aspects, the suppressor is employed for the treatment of an individual infected with HIV virus or with AIDS.

Description

[0001] This application claims priority to US Provisional Patent Application No. 60 / 812,685, filed June 9, 2006, which is hereby incorporated by reference in its entirety. [0002] Statement regarding this invention as a federally funded research project: This invention was supported by the National Institutes of Health (NIH) under grant numbers NIH NIGM R01 GM63080 and N1H-N1AID R01AI40891. The United States Government has certain rights in this invention. technical field [0003] The present invention at least relates to the fields of cell biology, molecular biology and medicine, and particularly relates to a pharmaceutical composition for treating HIV / AIDS. Background technique [0004] HIV / AIDS remains an incurable disease that threatens the lives of millions of people around the world. Cocktail therapy, also commonly known as HAART or ART, is currently the most successful antiretroviral therapy that can reduce a patient's HIV-1 to levels below detectable levels. Typic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/37C12Q1/02
CPCG01N2333/16C12Q1/18
Inventor 赵玉琪罗伯特·T·艾德
Owner SUZHOU SIRNAOMICS BIOPHARMACEUTICALS CO LTD
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