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Sample pretreatment integrated chip

A sample pretreatment and integrated chip technology, which is applied in the preparation of test samples, material inspection products, biological testing, etc., can solve the problems of low integration, low level of pretreatment, and single function of preprocessing chips, and improve Mixing efficiency, reduce filter cake effect, avoid clogging effect

Inactive Publication Date: 2012-06-27
INST OF ELECTRONICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current sample pretreatment chip has a single function, low pretreatment level, and low integration. Most of them cannot be directly used for subsequent biochemical reactions and analysis and detection.

Method used

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  • Sample pretreatment integrated chip
  • Sample pretreatment integrated chip
  • Sample pretreatment integrated chip

Examples

Experimental program
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Effect test

Embodiment 1

[0058] The sample pretreatment integrated chip based on MEMS technology mainly includes three sample pretreatment functions: cell separation, cell rupture and DNA purification. The dam-type cross-flow filtration microstructure is designed for size difference, which realizes the continuous separation and online collection of red blood cells and white blood cells during the flow of blood samples; based on the chemical method of breaking cells, the sandwich-type micro-channel network structure is designed to realize the chemical reagents and cells. Quickly mix to finally realize the dissolution and rupture of cells; extract genomic DNA based on the principle of solid phase extraction, and use MEMS technology to prepare integrated solid phase carriers in the chip to achieve rapid extraction of DNA genes in samples. In addition, the red blood cells and plasma exported through the red blood cell outlet can also be integrated with other subsequent biochemical reactions, separation and...

Embodiment 2

[0066] The sample pretreatment integrated chip based on MEMS technology mainly includes two sample pretreatment functions: cell rupture and DNA purification. Mixing, and finally achieve the dissolution and rupture of cells; extract genomic DNA based on the principle of solid phase extraction, and use MEMS technology to prepare integrated solid phase carriers in the chip to achieve rapid extraction of genes in samples.

[0067] The size of the sample pretreatment integrated chip is 4cm × 1.2cm × 5mm (length × width × height), and the diameter of the sample inlet and sample outlet holes is 2mm, including the cover sheet a and the structure sheet b, such as Figure 3A shown. Cover sheet a is a glass sheet, which is used to seal the chip, and includes three through holes: through hole 38, through hole 32 and through hole 33, such as Figure 3A shown. Structural sheet b is a silicon sheet, including mixing and purification channel 37 (for cell rupture and DNA purification) and th...

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Abstract

The invention relates to a sample pretreatment integrated chip which comprises a cover plate and at least one layer of structure plate, wherein the structure plate is provided with micro grooves and at least three holes communicated with the micro groove; and the micro grooves are at least two of the following grooves: a separation groove having a micro structure of cross-flow separation or cross-flow filtration, a purifying groove having a solid phase extraction micro structure and a mixed groove formed by converging at least two secondary grooves. The sample pretreatment integrated chip hasthe pretreatment functions of separation, filtration, mixing, purifying, and the like, is used for the pretreatment of samples of blood, urine, and the like and gains analysis samples of DNA, protein, amino acid, and the like which satisfy the follow-up detection need. The sample pretreatment integrated chip separates or filters granules or cells on the basis of the principle of cross-flow separation or cross-flow filtration, ruptures cells on the basis of the principle of a chemical method and purifies target molecules on the basis of the principle of solid phase extraction. The invention develops the integrated micro-fluidic chip for the pretreatment of multiple samples by adopting the micro-electromechanical system technology, has at least two sample pretreatment functions and has continuous and efficient sample pretreatment effect.

Description

technical field [0001] The invention relates to the technical field of microfluidic biochips, and is a sample pretreatment chip integrating pretreatment functions such as separation or filtration, mixing, and purification. Specifically, cross-flow separation or cross-flow filtration is used for separation or filtration. The optimized design of the microchannel network is used for liquid mixing, and the solid phase extraction method is used for the purification of target molecules. Finally, at least two of the above pretreatment functions are integrated on the microfluidic chip by using MEMS technology. Background technique [0002] With the intersecting research between MEMS technology and analytical science, computer science and life science, microfluidic biochips have begun to develop into micrototal analysis systems (μTAS). μTAS refers to the integration or basic integration of the basic operation units involved in the fields of biology and chemistry, such as sample prepa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N33/48
Inventor 崔大付陈兴刘长春张璐璐
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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