Application of clam active polypeptide in preparing anti-lung cancer medicament

A technology of anti-lung cancer drugs and active peptides, applied in anti-tumor drugs, drug combinations, pharmaceutical formulations, etc., can solve problems that have not been reported

Inactive Publication Date: 2009-08-05
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the research on the effect of clam polypept...

Method used

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  • Application of clam active polypeptide in preparing anti-lung cancer medicament
  • Application of clam active polypeptide in preparing anti-lung cancer medicament
  • Application of clam active polypeptide in preparing anti-lung cancer medicament

Examples

Experimental program
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Embodiment Construction

[0012] The following embodiments will further illustrate the present invention in conjunction with the accompanying drawings.

[0013] The clam active polypeptide of the present invention can adopt the following separation and purification methods:

[0014] 1) Freeze the fresh clams for 2 hours, the freezing temperature can be -20°C, take out the clam meat, add water, according to the mass ratio, clam meat: water is 1:1, homogenize 3 times in a high-speed tissue grinder, 30s each time , to obtain homogenate.

[0015] 2) Centrifuge at 15000g×30min to obtain the supernatant, salt out with 20% ammonium sulfate at 4°C overnight, centrifuge at 15000g×30min to obtain the supernatant, and obtain the first extract.

[0016] 3) Add 4 times the volume of ethanol to extract overnight, centrifuge at 15000g×30min to obtain the supernatant, and obtain the second extract.

[0017] 4) After the ethanol in the second extract was removed by rotary evaporation at 40°C, freeze-dried and concent...

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Abstract

The invention relates to clam active polypeptide, in particular to application of clam active polypeptide in preparing an anti-lung cancer medicine. The invention provides the application of the clam active polypeptide in preparing the anti-lung cancer medicine. As shown by experimental results by an MTT method and a cell counting method, the clam polypeptide has specific effect of inducing and killing lung cancer cells A549, and has obvious effect of inhibiting the lung cancer cells A549, so that the clam active polypeptide can be used for preparing the anti-lung cancer medicine.

Description

technical field [0001] The invention relates to a clam active polypeptide, in particular to the application of a clam active polypeptide in the preparation of anti-lung cancer drugs. Background technique [0002] The method of promoting apoptosis will be one of the main ways of tumor treatment. Therefore, finding drugs that can effectively induce apoptosis or damage cancer cells has become a hot spot in current research. On the other hand, continuous cell division and continuous proliferation are important characteristics of cancer cells, and the investigation of the inhibitory effect on the proliferation of cancer cells is a major indicator for identifying the anti-tumor effect of exogenous substances. Meretrix meretrix is ​​not only delicious and nutritious, but also has extremely high medicinal value [Ding Yuan, Ding Wei, Zhang Jide. List of medicinal marine shellfish in my country and its application [J]. Marine Medicine, 1984, 3: 34]. [0003] Modern studies have show...

Claims

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Application Information

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IPC IPC(8): A61K35/56A61K38/17A61P35/00A61K35/618
Inventor 康劲翮陈清西
Owner XIAMEN UNIV
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