Preparation method of fish maw protein beverage
A technology of protein beverage and swim bladder, which is applied in fish protein components, food preparation, protein food components, etc. It can solve the problem of bitter taste of fish swim bladder protein degradation products and insufficient attention to fishy smell, so as to improve nutrition and function Features, broad market prospects, and mild effects
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Embodiment 1
[0017] 1. Preparation of swim bladder slurry. Take 0.5kg of small, low-value dry eel maw without pests and mildew, and perform routine cleaning and crushing; soak in drinking water 10 times the weight of the swim bladder for 60 minutes; add 20kg of drinking water according to the solid-to-liquid ratio of 1:50, and stir , Homogenizing to obtain swim bladder slurry;
[0018] 2. Enzymatic hydrolysis of swim bladder. Add 0.5% flavor protease (3.0×10 4 u / g, Novo Company), under the conditions of pH 6.8 and temperature 55°C, enzymolysis was performed for 3 hours; the temperature was raised to 90°C, and the enzyme was inactivated for 9 minutes; centrifuged at 4000 rpm for 20 minutes to obtain the supernatant;
[0019] 3. Debittering and deodorization of enzymatic hydrolyzate. Add 1.0% white sugar, 0.2% citric acid, and 1.5% yeast to the supernatant, and incubate at pH 6.0 and 34°C for 45 minutes; then, add 1.0% β-cyclodextrin, Incubate for 55 minutes; centrifuge at 8000rpm for 6 ...
Embodiment 2
[0023] 1. Preparation of swim bladder slurry. Take 0.3kg of sharp fish maw without pests and mildew, and carry out routine cleaning and crushing; soak in drinking water 12 times the weight of fish maw for 80 minutes; add 12.9kg of drinking water according to the solid-liquid ratio of 1:55, stir and homogenize fish maw slurry;
[0024] 2. Enzymatic hydrolysis of swim bladder. Add 0.75% flavor protease (3.0×10 4 u / g, Novo Company), at a pH value of 6.8 and a temperature of 55°C, enzymolysis was performed for 4 hours; the temperature was raised to 90°C, and the enzyme was inactivated for 9 minutes; centrifuged at a speed of 4500rpm for 15 minutes to obtain a supernatant;
[0025] 3. Debittering and deodorization of enzymatic hydrolyzate. Add 1.5% white sugar, 0.3% citric acid, and 2.5% yeast to the supernatant, and incubate and react at pH 5.8 and 37°C for 60 minutes; then, add 2.0% β-cyclodextrin, Keep warm for 65 minutes; centrifuge at 8000rpm for 10 minutes, and the obtain...
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