Capillary array analyzer

Abstract

The invention belongs to the technical field of bioanalysis, and relates to a capillary array analyzer, wherein, exciting light sent out by a light source is reflected to a vibrating mirror by a reflecting mirror and a fluorescence separating mirror; as the vibrating swings around a rotating shaft in a reciprocating manner, the exciting light also swings in a reciprocating manner; then the exciting light arrives at a scanning objective; the exciting light is focused on a focal plane for accommodating a capillary array by the scanning objective and is converted into one-dimensional translational scanning on the focal plane by the scanning objective; during the scanning process, samples marked with fluorescent substances in capillaries are excited by the exciting light in sequence; fluorescence emitted by the fluorescent substances marked on the samples is collected by the scanning objective and converted into a parallel beam that is coaxial with the exciting light and spreads in the opposite direction; the parallel beam is separated from the exciting light through reflection and then arrives at a convergent lens; and the parallel beam of fluorescence, from which the background light is filtered by a fluorescence color filter on a color wheel, is detected by a photodetector after passing through a confocal ostiole. The capillary array analyzer provided by the invention has the advantages of simple structure, small size, low cost and excellent performance.

Description

technical field [0001] The invention belongs to the technical field of biological analysis, in particular to a capillary array analyzer. Background technique [0002] Capillary electrophoresis is to make the sample migrate in the capillary through the action of the electric field, and different components in the sample have different migration speeds, so as to achieve separation, and detect at the detection window in the capillary, and finally detect the sample through the obtained detection signal for analysis. This technology is widely used in biochemical analysis, such as DNA and protein separation analysis, which has high separation efficiency, low sample consumption and high degree of automation. It is widely used in DNA sequencing, biological and clinical research, forensic identification, environmental protection, analytical chemistry and Food testing and other fields have a wide range of applications. The detection of electrophoretic samples mainly includes isotope...

AI Technical Summary

Problems solved by technology

This spectroscopic detection method firstly requires many photodetectors, which increases the production cost and the volume of the detector; secondly, due to the large number of color filters and the loss of fluorescence, the detection sensitivity is limited; finally, due to the multi-color filter configuration in In the optical path, therefore, the space requirement is large, further increasing the volume of the detector

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