Kalium ion diagnosis/measuring reagent kit and kalium ion concentration determination method
A kit and ion technology, applied in the field of potassium diagnosis/determination kits and determination of potassium concentration, can solve the problem of expensive electrodes
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Embodiment 1
[0048] The potassium (ion) diagnosis / measurement reagent of the present embodiment is a single reagent, comprising:
[0049] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0050] Stabilizer 500mmol / L
[0051] Reduced coenzyme 0.25mmol / L
[0052] Pyruvate kinase 6000U / L
[0053] Pyruvate dehydrogenase 8000U / L
[0054] Aldehyde dehydrogenase 10000U / L
[0055] Adenosine diphosphate 3mmol / L
[0056] Phosphoenolpyruvate 3mmol / L
[0057] Ferricytochrome b1 4mmol / L
[0058] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.
[0059] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested potassium (ion) sample to the reagent is 1 / 25, the reaction direction is negative reaction...
Embodiment 2
[0062] The potassium (ion) diagnosis / measurement reagent of the present embodiment is a double reagent, comprising:
[0063] Reagent 1
[0064] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0065] Stabilizer 50mmol / L
[0066] Reduced coenzyme 0.25mmol / L
[0067] Adenosine diphosphate 3mmol / L
[0068] Phosphoenolpyruvate 3mmol / L
[0069] Ferricytochrome b1 4mmol / L
[0070] Reagent 2
[0071] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0072] Stabilizer 500mmol / L
[0073] Pyruvate kinase 6000U / L
[0074] Pyruvate dehydrogenase 8000U / L
[0075] Aldehyde dehydrogenase 10000U / L
[0076] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.
[0077] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test subwavelength 405nm, tested potassium (ion) s...
Embodiment 3
[0080] The potassium (ion) diagnosis / measurement reagent of the present embodiment is three reagents, comprising:
[0081] Reagent 1
[0082] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0083] Stabilizer 50mmol / L
[0084] Reduced coenzyme 0.25mmol / L
[0085] Adenosine diphosphate 3mmol / L
[0086] Phosphoenolpyruvate 3mmol / L
[0087] Ferricytochrome b1 4mmol / L
[0088] Reagent 2
[0089] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0090] Stabilizer 500mmol / L
[0091] Pyruvate dehydrogenase 8000U / L
[0092] Aldehyde dehydrogenase 10000U / L
[0093] Reagent 3
[0094] Tris(carboxymethyl)aminomethane—hydrochloric acid buffer 100mmol / L
[0095] Stabilizer 500mmol / L
[0096] Pyruvate kinase 6000U / L
[0097] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.
[0098] When measuring potassium (ion) concentration, set on the automatic b...
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