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Phosphorescent chemical sensor for qualitatively detecting contrast of aminothiopropionic acid and homocysteine and use thereof

A photochemical sensor, homocysteine ​​technology, applied in chemical instruments and methods, organic chemistry, fluorescence/phosphorescence, etc., to achieve the effect of eliminating interference and high detection sensitivity

Inactive Publication Date: 2009-06-10
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to use a kind of iridium complex as the ratio phosphorescence chemical sensor to qualitatively detect the mercapto amino acid in the semi-aqueous solution, to solve the technical problem of rapid real-time detection of the mercapto amino acid

Method used

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  • Phosphorescent chemical sensor for qualitatively detecting contrast of aminothiopropionic acid and homocysteine and use thereof
  • Phosphorescent chemical sensor for qualitatively detecting contrast of aminothiopropionic acid and homocysteine and use thereof
  • Phosphorescent chemical sensor for qualitatively detecting contrast of aminothiopropionic acid and homocysteine and use thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Preparation of iridium complex: weigh IrCl 3 ·3H 2 O (5.52mmol) and the corresponding cyclometalated C^N ligand 4-(2-pyridine)-benzaldehyde (1.04mmol) were added to the double-necked flask, and vacuum-filled with nitrogen-vacuumized on the double-row tube , cycle three times, and finally protect the reaction system with nitrogen. After injecting a mixture of 2-ethoxyethanol and water (3:1, v / v) with a syringe, the reaction mixture was heated to 10°C, stirred for about 24 hours, and a precipitate formed. After the reaction was stopped, the reaction mixture was cooled to room temperature, and a precipitate was obtained by filtration. The resulting precipitate was washed with water and ethanol respectively to obtain a red solid iridium dichloro bridge compound (pba) 2 Ir(μ-Cl) 2 Ir(pba) 2 . Weigh iridium dichloride bridge compound (0.16mmol) and bipyridine ligand (0.32mmol) and join in the double-necked flask, vacuumize-fill nitrogen-vacuumize on the double-row tube,...

Embodiment 2

[0024] The ultraviolet spectrum test of the response of the complex to mercapto amino acids: the iridium complex was dissolved in the buffer solution of DMSO and HEPES, the pH value was 7.2, the volume ratio of the two was 9:1, the concentration of HEPES was 50mmol / L, and the The concentration is 20 μmol / L, and then add homocysteine ​​and cysteine ​​solutions prepared in an equal volume of 0-120eq HEPES buffer. After equilibration, measure the UV spectrum, see figure 1 .

Embodiment 3

[0026] Phosphorescence spectroscopic test of the complex’s response to mercapto amino acids: the iridium complex was dissolved in a buffer solution of DMSO and HEPES, the pH value was 7.2, the volume ratio of the two was 9:1, and the concentration of HEPES was 50mmol / L. The concentration is 20 μmol / L, and then add homocysteine ​​and cysteine ​​solutions prepared in an equal volume of 0-120eq HEPES buffer. After equilibration, measure the phosphorescence spectrum, see figure 2 .

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Abstract

The invention relates to the field of phosphorescence chemical sensor technology, specifically to a scale phosphorescence chemical sensor for qualitatively detecting cysteine and homocysteine and application thereof, characterized by relating to an iridium complex containing aldehyde group. The aldehyde group of the iridium complex generates cyclization reaction with amido and sulfhydryl of homocysteine and cysteine to convert to heterocycle, aldehyde group and benzene ring contained in original iridium complex conjugate, then conjugation of aldehyde group and benzene ring disappears after reacting with cysteine or homocysteine, thereby affecting spectroscopic properties of the generated products, and detecting cysteine and homocysteine through using the property. The inventive iridium complex solution emits yellow phosphorescence when being excitated by 365 nm ultraviolet light, and emits red phosphorescence reacting with mercaptoamino acid, without being interrupted by other amino acid.

Description

technical field [0001] The invention relates to the technical field of phosphorescent chemical sensors, in particular to a specific phosphorescent chemical sensor for qualitatively detecting cysteine ​​and homocysteine ​​and its application. Background technique [0002] Thiol amino acids include homocysteine ​​and cysteine, both of which have important physiological functions. The clinical application of homocysteine ​​is mainly used as a risk indicator for cardiovascular diseases, especially coronary atherosclerosis and myocardial infarction, and its concentration is directly proportional to the risk of the disease. Cysteine ​​is involved in the reduction process of cells and phospholipid metabolism in the liver. It can protect liver cells from damage and promote liver function. Due to these important physiological functions, the detection of sulfhydryl-containing amino acids has received great attention. The current detection methods mainly include chromatographic separ...

Claims

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Application Information

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IPC IPC(8): G01N21/64C07F15/00
Inventor 李富友刘倩陈绘丽黄春辉
Owner FUDAN UNIV
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