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Simple cell climbing film culture and experimental apparatus

An experimental device and cell technology, applied in tissue cell/virus culture devices, biochemical instruments, biochemical equipment and methods, etc., can solve problems such as difficulty in obtaining experimental data, difficult operation of cover glass, and low success rate of experiments. Achieve the effects of less cell demand, easy labeling, and high experimental success rate

Inactive Publication Date: 2011-04-20
ANHUI PROVINCIAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1. There is no dedicated experimental device for climbing slices, and each experiment is to place the climbing slices on the bottom of the petri dish / plate, and use this as a container to add culture medium or drugs that can submerge the climbing slices, and the demand for liquid is large; At the same time, due to the large surface area of ​​the culture dish / plate, the liquid is easy to volatilize, it is difficult to obtain accurate experimental data, affect the growth of cells, and require a large amount of culture medium and drugs
[0005] 2. Traditional slides need to cut the cover glass suitable for the well plate or purchase special cell slides. When taking out the slides, the slides are often damaged
[0006] 3. The cover glass is difficult to operate, which limits the number of each experiment and makes it difficult to conduct experiments in large quantities
[0007] 4. In further experiments (such as immunohistochemical experiments) on the coverslip slides, the coverslip is easily damaged, difficult to operate, and the success rate of the experiment is low

Method used

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  • Simple cell climbing film culture and experimental apparatus
  • Simple cell climbing film culture and experimental apparatus
  • Simple cell climbing film culture and experimental apparatus

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Embodiment Construction

[0028] see figure 1 , figure 2 and image 3 , the structural settings of this embodiment include:

[0029] A base 1 has a rectangular groove 11 for horizontally placing the glass slide 2, and a through hole 13 corresponding to the center of the slide glass 2 is provided on the base bottom plate 12; it is located above the rectangular groove 11 and is symmetrical to the base 1 A horizontal bayonet 14 is arranged on the two side walls of the base 1 , and a through groove 15 communicating with the horizontal bayonet 14 is provided on the surface of the base 1 .

[0030] A pipe body 3, a sealing ring 6 is provided at the bottom of the pipe body, and a pair of side ears 31 are arranged symmetrically on both sides of the bottom mouth of the pipe body. In the bayonet 14, a seal is formed between the bottom opening of the tube body and the slide glass 2; the through hole 13 on the base bottom plate 12 is at the position of the bottom opening of the tube body; Cap 4.

[0031] The...

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Abstract

The invention relates to a simple cell climbing film culture and experimental apparatus. The invention is characterized in that the simple cell climbing film culture and experimental apparatus comprises a base with a rectangular groove for horizontally placing a glass slide, horizontal bayonets positioned above the rectangular groove and arranged in two side walls corresponding to the base, and atube body, wherein, a through hole is formed on a bottom plate of the base and in the center position corresponding to a glass slide; through grooves communicated with the horizontal bayonets are formed on the surface of the base; a sealing ring is arranged at the bottom opening of the tube body, a pair of side lugs is symmetrically arranged on both sides of the bottom opening of the tube body, the pair of the side lugs can pass through the through grooves and can be rotationally inserted in the horizontal bayonets of the base, and sealing is formed between the bottom opening of the tube bodyand the glass slide; and the through hole on the bottom plate of the base is positioned in the position where the bottom opening of the tube body is. The invention has the advantages of convenient operation, portability, compactness and high success ratio of experiment.

Description

technical field [0001] The invention relates to an experimental vessel, more specifically a device for cell culture experiments. Background technique [0002] Life science is an experimental science, and many of its achievements are discovered and developed through experiments. As one of the indispensable and commonly used experimental methods in various fields of life sciences, in vitro cell culture has an unshakable position. Its outstanding feature is to observe and study the laws of cell life activities under in vitro conditions, and the cell culture is not affected by the complex environment in the body, and the culture conditions can be artificially changed (such as changes in external factors such as physics, chemistry, biology, etc.). Further observe the physiological function changes of cells under the influence of single factor or multiple factors. Many important advances in various research fields of life sciences and the formation of new concepts often come fro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00
Inventor 凌斌周颖卫莹李敏冯定庆
Owner ANHUI PROVINCIAL HOSPITAL
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